Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain.

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Date
1994-05-01
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Abstract
RNA isolation from purified cucumber mosaic virus, CMV-U strain and cDNA synthesis was carried out. The coat protein gene (RNA4) region was amplified selectively by polymerase chain reaction (PCR) with CMV RNA4-specific primers. Double-stranded cDNA was cloned in PRT103 vector at Xho1/Kpn1 site and about 1kb insert obtained. The insert was partly sequenced which showed 50% sequence homology with CMV-Q, C and WL strains.
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Raj SK, Leiser RM, Plobner L. Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain. Indian Journal of Experimental Biology. 1994 May; 32(5): 345-7
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https://imsear.searo.who.int/handle/123456789/56832
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Indian Journal of Experimental Biology