Sensitivity of Salmonella and Shigella to Mangifera indica Linn (Mango) Crude Leaf Extracts.
Loading...
Date
2014-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Aims: To study the antimicrobial activities of methanol, acetone and aqueous extracts of
Mangifera indica Linn crude leaves on Salmonella and Shigella in order to provide natural
therapy against them.
Study Design: Salmonella and Shigella isolates were obtained from twenty-five stool
samples of hospital patients. Six isolates were identified and characterized. The six
isolates were labelled accordingly and used in the study. The antimicrobial activities of the extracts preliminary screened for the presence of phytochemical compounds were tested
on the six isolates.
Place and Duration of Study: The study was carried out in the Department of
Microbiology, Delta State University, Abraka, Nigeria between October, 2012 and August,
2013.
Methodology: The test isolates (Salmonella and Shigella) were isolated from stool
samples on Salmonella/Shigella agar and characterized. The phytochemical constituents
of the leaves of M. indica were extracted by soaking in water, methanol and acetone. The
extracts were investigated for antimicrobial activities on theisolates using the agar well
diffusion method.
Results: Phytochemical analysis of the leaf extract showed that the extracts contained
alkaloids, tannins, flavonoids, steroids, anthroquinones, reducing sugars, glycosides and
phenols. The acetone and methanol extracts exerted the highest zone of inhibition
(18.00mm and 15.00mm respectively) though there was a significant difference (p<0.05) in
the activities of the extracts. There was no significant difference (p>0.05) when compared
with that of the water extract. Salmonella was more susceptible to acetone extract than
Shigella with a Minimum Inhibitory Concentration of 25.00 mg/ml while that on Shigella was
50.00mg/ml. The MIC of the ethanolic extract on Salmonella was 50.00mg/ml while that of
Shigella was 100.00mg/ml.
Conclusion: The acetone and methanolextracts of the leaves of M. indica had high
inhibitory effects on Salmonella and Shigella thus providing a basis for its recommendation
in the treatment of diseases caused by these organisms.
Description
Keywords
Mangifera indica L., acetone, methanol, extracts, Salmonella
Citation
Okoko F J, Akpomie O O, Ikejiofor A G. Sensitivity of Salmonella and Shigella to Mangifera indica Linn (Mango) Crude Leaf Extracts. British Microbiology Research Journal. 2014 Dec; 4(12): 1521-1530.