Rapid micropropagation of Cucumis sativus var. Dastgerdi (Iranian cultivar) by Node Culture Technique.
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Date
2014-06
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Abstract
Tissue culture can be used in developing an efficient method for production of valuable
and enduring indigenous cultivars. A short-term protocol for plantlet proliferation by using
nodal segments was developed for Cucumis sativus, local cultivar from Isfahan; known as
Dastgerdi as a tolerant cultivar to the root-knot nematode. Nodal explants were cultured
on MS medium containing various concentrations of KIN (0, 0.5, 1 and 1.5mg.l-1) in
combination with IBA (0, 0.025 and 0.5mg.l-1). The best response for proliferation rate
(100%), mean number of nodes per plantlet (6.01) and percentage of rooted In vitro
propagated shoots were observed on medium supplemented by KIN (1.5mg.l-1) after
about 3-4 weeks. The maximum length (5.43cm) of micro-shoots obtained on MS medium
with KIN (1mg.l-l) + IBA (0.025mg.l-1).
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Keywords
Cucumis sativus, IBA, KIN;, native cultivar, proliferation
Citation
Mahmoud Otroshy, Arash Mokhtari. Rapid micropropagation of Cucumis sativus var. Dastgerdi (Iranian cultivar) by Node Culture Technique. British Biotechnology Journal. 2014 Jun; 4(6): 733-739.