Influence of clove oil on certain quorum-sensing-regulated functions and biofilm of Pseudomonas aeruginosa and Aeromonas hydrophila.
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Date
2013-12
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Abstract
Quorum sensing (QS) plays an important role in virulence, biofilm formation and survival of many pathogenic
bacteria including Pseudomonas aeruginosa. This signalling pathway is considered as novel and promising target for
anti-infective agents. In the present investigation, effect of the Sub-MICs of clove oil on QS regulated virulence
factors and biofilm formation was evaluated against P. aeruginosa PAO1 and Aeromonas hydrophila WAF-38 strain.
Sub-inhibitory concentrations of the clove oil demonstrated statistically significant reduction of las- and rhl-regulated
virulence factors such as LasB, total protease, chitinase and pyocyanin production, swimming motility and
exopolysaccharide production. The biofilm forming capability of PAO1 and A. hydrophila WAF-38 was also reduced
in a concentration-dependent manner at all tested sub-MIC values. Further, the PAO1-preinfected Caenorhabditis
elegans displayed an enhanced survival when treated with 1.6% v/v of clove oil. The above findings highlight the
promising anti-QS-dependent therapeutic function of clove oil against P. aeruginosa.
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Keywords
Biofilm, C. elegans, clove oil, quorum-sensing inhibition
Citation
Husain Fohad Mabood, Ahmad Iqbal, Asif Mohammad, Tahseen Qudsia. Influence of clove oil on certain quorum-sensing-regulated functions and biofilm of Pseudomonas aeruginosa and Aeromonas hydrophila. Journal of Biosciences. 2013 Dec; 38(5): 835-844.