Biosynthesis of eukaryotic cell surface glycosphingolipids using solubilized glycosyltransferases.
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Date
1985-08
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Abstract
Two fucsyltransferases (FucT-2 and FucT-3) have been solubilized from Golgi-rich
membrane fraction of bovine spleen, using a cationic detergent. FucT-3 was distinguished
from FucT-2 by comparing their kinetic parameters and heat stability. FucT-2 and FucT-3 lost
activity (85 %) and (5 %), respectively, when heated at 55°C for 10 sec. Two galactosyltransferases
(GalT-3 and GalT-4) and two sialyltransferases (SAT-2 and SAT-3) have also been
solubilized from embryonic chicken brain membranes using nonionic detergents. Affinity
chromatography and microisoelectric focusing were used to separate these enzymes into
functionally pure fractions. Anomeric and positional linkages in some of the products (LM1
and LD1c) have also been established. The terminal NeuAc(α2–8) linkage in GD3 and LD1c
was established by identification of the partially methylated penultimate [Ac-14C]sialic acid.
Description
Keywords
Fucosyltransferase, glycosphingolipids, gangliosides, glycosyltransferase, galac tosyltransferase, sialyltransferase
Citation
Basu Subash, De Tripti, Kyle John W, Basu Manju. Biosynthesis of eukaryotic cell surface glycosphingolipids using solubilized glycosyltransferases. Journal of Biosciences. 1985 Aug; 8(1&2): 413-424.