Characterisation of rinderpest virus RNA and the action of actinomycin D on its replication.
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Date
1979-09
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Abstract
RNA extracted from purified rinderpest virus was characterised by
sucrose gradient sedimentation and polyacrylamide gel electrophoresis. The predominant
virion RNA species had a sedimentation constant of 46S and its estimated molecular
weight was 4·8 × 106 daltons. Consistently high amounts of UMP and AMP
were detected. The melting-temperature profile of the virion RNA suggested absence
of secondary structure.
The effect of actionomycin D on the replication of rinderpest virus in Vero cells
was studied by following the viral RNA synthesis using labelled uridine as well as by
infectivity titration. The viral RNA synthesis was not affected until 12 h following
infection and was inhibited thereafter between 18 and 48 h to an extent of 25% at
5 and 10 μg levels of the drug. A 100 to 1000-fold reduction in the infectivity
titres was observed in the presence of the drug. These results suggest that actinomycin
D inhibits rinderpest viral RNA replication. Sedimentation analysis of viral RNA
extracted from drug-treated cultures showed inhibition of the genome RNA of rinderpest
virus.
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Keywords
Rinderpest virus RNA, viral RNA synthesis in infected cells, infectivity titration, sedimentation analysis
Citation
Prakash K, Antony A, Ramakrishnan T. Characterisation of rinderpest virus RNA and the action of actinomycin D on its replication. Journal of Biosciences. 1979 Sept; 1(3): 307-316.