Flavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf.

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Date
2015-04
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NISCAIR-CSIR, India
Abstract
In this study, we report isolation of flavonoids, viz., 3-O-methylquercetin, tangeritin, luteolin-7-O-glucoside, luteolin, apigenin-7-O-glucoside, apigenin-8-C-glucoside, luteolin-8-C-glucoside, luteolin-6-C-glucoside, diosmetin and catechin from the methanolic extract of Hyparrhenia hirta employing high performance liquid chromatography and liquid chromatography-electrospray ionization-tandem mass spectrometry. The total phenolic content of H. hirta extract was 105.58 ± 0.1 mg gallic acid equivalents/g of plant extract while the total flavonoid content was 45.20 ± 0.2 mg quercetin equivalents/g of plant extract and the total condensed tannin were 72.35 ± 0.7 mg catechin equivalents/g of plant extract by reference to standard curve. The antioxidant activity was assayed through the antioxidant capacity by phosphomolybdenum assay, the reducing power assay and the radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl method. The extract showed dose dependant activity in all the three assays.
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Keywords
Apigenin, Catechin, Diosmetin, HPLC, LC-ESI-MS, Luteolin, Methylquercetin, Tangeritin, Thatching grass
Citation
Bouaziz-Ketata Hanen, Zouari Nabil, Salah Hichem Ben, Rafrafi Moez, Zeghal Najiba. Flavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf. Indian Journal of Experimental Biology. 2015 Apr; 53(4): 208-215.