Antilithiatic activity of poly-herbal formulation tablets by in-vitro method.
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Date
2013-05
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Abstract
Hydroalcoholic extracts of Kalanchoe pinnata and Rotula aquatica and its combination were formulated herbal
tablets, evaluated for antilithiatic in vitro method. The homogenous precipitation method was used. The study
was carried out in glass tubes. The buffer system used was TRIS buffer pH 7.4. The experiment consists of the
following tubes for control and test, 25 ml each of 25 mM CaCl2. 2H2O, 25 mM Na2HPO4. 2H2O or 25mM
Na2C2O4. To the tubes of each set, tablet formulation or an equal amount of vehicle was added. The tubes were
incubated at 37°C for 4 h. The precipitate of calcium phosphate was generated by mixing 1 ml of solution from
the tubes having calcium chloride dihydrate and disodium hydrogen phosphate monohydrate and Calcium oxalate
precipitate was generated by mixing 1 ml of solutions from the tubes having calcium chloride dihydrate and
sodium oxalate solutions. Calcium was estimated using titrimetry and phosphorus was estimated using
colorimetric analysis. Appropriate standard curves were done with each set of experiments. The amounts of
precipitate of calcium and phosphate were determined in each of the respectively. The percent inhibition of the
test was calculated in comparison with the control samples. Herbal tablet formulation showed antilithiatic activity
to the marketed formulation in terms of inhibiting the formation of phosphate precipitate but showed a
significantly better potential in preventing the formation of the calcium precipitate. The herbal tablet formulation
of Kalanchoe pinnata and Rotula aquatica have inhibitory effect on calcium oxalate crystallization thus may be
beneficial in the treatment of renal lithiasis.
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Keywords
Antilithiatic activity, Kalanchoe pinnata, Rotula aquatica, Herbal tablet Formulation, In-vitro
Citation
Gilhotra Umesh Kumar, Mohan Govind, Christina A J M. Antilithiatic activity of poly-herbal formulation tablets by in-vitro method. Journal of Applied Pharmaceutical Science. 2013 May; 3(5): 43-48.