Inhibitory effect of antioxidants on the benz[a]anthracene-induced oxidative DNA damage in lymphocyte.
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Date
2011-01
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Abstract
Benz[a]anthracene is a ubiquitous environmental contaminant formed during the incomplete combustion of
organic material. Some of the metabolites of benz[a]anthracene are known to be toxic and carcinogenic. In this
investigation, benz[a]anthracene-induced oxidative damage to lymphocyte DNA was evaluated with the Comet
assay (single cell gel electrophoresis). The level of oxidative DNA damage caused by benz[a]anthracene
increased in a dose-dependent manner (24, 49) and oxidative DNA damage was significantly inhibited by 5 and
10 μg ml-1 ascorbate, 5 μg ml-1 polyphenols, as well as 5 and 10 μg ml-1 curcumin. Moreover, traditional Korean
medicinal herbs such as Acanthopanax and ginseng significantly reduced DNA damage. The results demonstrate
that antioxidant supplementation to lymphocytes inhibits oxidative DNA damage in vitro, supporting an inhibitory
effect against oxidative DNA damage, probably due to reduction of reactive oxygen species production induced
by benz[a]anthracene.
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Keywords
Benz[a]anthracene, Comet assay, Oxidative DNA damage, Antioxidant
Citation
Seo Yoo-Na, Lee Mi-Young. Inhibitory effect of antioxidants on the benz[a]anthracene-induced oxidative DNA damage in lymphocyte. Journal of Environmental Biology. 2011 Jan; 32(1): 7-10.