European Journal of Medicinal Plants

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https://imsear.searo.who.int/handle/123456789/151205

Chief Editors: Dr. Marcello Iriti, Dr. Valentina Savo
ISSN: 2231-0894

Frequency: Quarterly

Language: English

Open Access Peer-reviewed journal

Web site: https://www.sciencedomain.org/journal-home.php?id=13

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Browsing European Journal of Medicinal Plants by Subject "ABTS"

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    GC-MS Analysis, Antioxidant and Cytotoxic Activities of Mentha spicata
    (Science Domain International, 2018-12) Abdel-Hady, Heba; El-Wakil, Eman Ahmed; Abdel-Gawad, Mahfouz
    Aims: Mentha spicata medicinal properties are well known. In this study, Total phenolic and flavonoid contents and the antioxidant activity of the methanol extract of Mentha spicata were determined as well as evaluation of the cytotoxic activity of it. Also, the identification of some bioactive compounds in the plant was analysed. Place and Duration of Study: Extraction and antioxidant at Medicinal Chemistry Department, Theodor Bilharz Research Institute, cytotoxic evaluation and GC-Ms analysis at Al-Azhar University, Egypt. Methodology: The antioxidant activity was determined by two methods, DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS (2-2՝azinobis (3-ethylbenzthiazoline-6-sulphonic acid) while, the cytotoxic assay was determined by MTT assay on HepG-2 (Human hepatocellular carcinoma) and MTC-116 (Human colon carcinoma). Regarding to the chemical identification of methanol extract was carried out by GC-MS analysis. Results: The study proved that Mentha spicata has high Total phenolic and flavonoid contents (388.20±2.38mg GAE/gm of extract &204.01±17.93mg RE/gm of extract) respectively. Also, exhibited promising antioxidant activity by DPPH & ABTS (IC50=65.13±1.29 µg/ml & 52.31±0.81 µg/ml) respectively. The methanol extract of the plant showed a good cytotoxic effect on HepG2 and HTC-116 (IC50=25.2±3.6µg/ml & 62.1±4.9µg/ml) respectively. GC-MS analysis of the methanol extract of Mentha spicata showed 43 oxygenated hydrocarbon compounds. The major ones are Hexadecanoic acid, methylEster (palmitic acid ester) (31.51%) followed by 9,12,15-Octadecatrienoic acid,methyl ester (CAS)(methyl linolenate) (22.10%), 2-Pentadecanone,6,10,14-trimethyl- (CAS)(6.82%), Phytol (6.20%), 9,12-Octadecadienoic acid(Z,Z)-, methyl ester (6.18%), Hexadecanoic acid (palmitic acid ) (5.95%) and Methyl stearate (4.49%). Conclusion: Mentha spicata is a potential antioxidant and anticancer agents.
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    Phytochemical Study and Evaluation of the Antioxidant Activity of Extracts of the Roots of Chrysopogon nigritanus
    (Sciencedomain International, 2023-03) Mbodj, M.; Gaye, A. A.; Samb, I.; Gaye, M. L.
    In this work, we were interested in a medicinal plant named Chrysopogon nigritanus, belonging to the Poaceae family. In order to carry out this study, we were interested first in the phytochemical screening test, after having carried out the extraction with two solvents (water and isopropanol), to characterize the different families of metabolites present in the extracts. Then, the assays of polyphenols and flavonoids were carried out in order to quantify the contents of these two compounds in each extract. Finally, the antioxidant activity of the extracts was evaluated by three methods: the CUPRAC method, the trapping of the 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH•) and the trapping of the ABTS•+ radical-cation (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)). The best antioxidant activities were obtained with the ABTS•+ radical scavenging with IC50 of 0.13 mg/mL for the aqueous extract and 0.19 mg/mL for the isopropanol extract. Based on these first results, an in-depth study is underway for various biological tests and the isolation of bioactive molecules.