Browsing by Author "Sharma, D K"

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    Acridine orange stain--a rapid method for diagnosis of neonatal septicemia.
    (1989-02-01) Gupta, S K; Sharma, U; Gupta, M L; Sharma, D K
    The present study included 150 newborns; 100 clinically septic and 50 clinically aseptic who served as control. Out of 100 clinically septic newborns, blood culture was positive in 33 (33%), serum CRP was positive in 64 (64%) and acridine orange stained buffy coat smear was positive in 76 (76%). Serum CRP was found to be the most specific (specificity 96%) and acridine orange stained buffy coat smear examination the most sensitive (sensitivity 94.3%) test for diagnosis of neonatal septicemia.
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    Anemia in childhood.
    (1970-07-01) Sharma, D K; Kalra, K; Thapar, R K; Agarwal, H C; Bansal, O P; Dayal, R S
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    Autoantibodies to intermediate filaments in acute viral hepatitis.
    (1986-09-01) Chandra, M; Golani, D D; Sachdeva, S; Bihari, N; Sharma, D K
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    Cardiovascular collapse following small dose of chloroquin in healthy young adult.
    (1986-07-01) Sogani, R K; Sharma, D K; Gupta, V
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    Effect of complete and skeleton photoperiods in baya weaver, Ploceus philippinus.
    (2006-07-04) Bhardwaj, Sanjay Kumar; Sharma, D K; Anushi,
    A study was performed to investigate the effects of complete and skeleton photoperiods on gonadal stimulation in baya weaver, Ploceus philippinus. In August 2003, five groups of acclimatized birds (n = 7 each) were exposed to 6L: 13D: 1L: 4D, 6L: 6D: 1L: 11D, 11L: 13D and 13L: 11D. Birds were refractory and their exposure to 6L:13D:1L:4D, 6L:6D:1L:11D, 11L:13D and 13L:11D did not evoke any response. This clearly indicates that the birds were insensitive to the stimulatory effects of these photoperiods and perceived them as short days. An attempt was made to test the responsivity by subjecting all these groups to long day exposure after 16 weeks of experiment. Under 16L: 8D, birds did not show a response during the first 4 weeks of exposure (week 16 to week 20), but thereafter there was a significant photoinduction except 11L:13D (week 24), but the rate and magnitude was different. Until the end of the experiment (32 weeks), all groups of birds had maximum testicular volume except 11L:13D. Birds pre-exposed to 6L:13D:1L:4D, 6L:6D:1L:11D, 11L:13D and 13L:11D responded to 16L:8D, which suggests that these photoperiods helped the birds in recovering their photosensitivity. The results from this experiment indicate that baya weaver interprets a light period depending when the light falls relative to its endogenous circadian system.
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    The effect of recent trauma on serum complement activation and serum C3 levels correlated with the injury severity score.
    (2004-07-24) Sharma, D K; Sarda, A K; Bhalla, S A; Goyal, A; Kulshreshta, V N
    PURPOSE: The objective of this prospective pilot study was to estimate serum C3 levels and complement activation within 12 hours of injury and to correlate them with abbreviated injury score (AIS) so as to predict the outcome of injury in terms of mortality or sepsis. METHODS: The test group (n=30) of patients with < 12 hours of injury were assessed by the AIS-80 and compared with a control group (n=10). Serum C3 levels were estimated by the single radial immunodiffusion technique and complement activation by both two-dimensional and counter immuno-electrophoresis. Statistical analysis was performed using the correlation regression analysis and the minimum discrimination information statistics (MDIS) analysis. RESULTS: Serum C3 levels were not reduced in patients with injury severity score (ISS) < 12 (n=7). There was a statistically significant difference of serum C3 levels in patients with ISS between 12 and 29 (n=15) and in patients with ISS more than 29 (n=9). Complement activation also correlated well with ISS (p < 0.05). Complement activation and serum C3 levels were found to influence subsequent development of septic complications in the post injury period, being significantly lower in patients who developed septic complications. Complement alterations in trauma patients were a direct result of the trauma since they were not seen in patients undergoing surgery. CONCLUSIONS: ISS is a good prognosticator of outcome of trauma and serum C3 levels and complement activation are good predictors for mortality and development of septic complications in recently injured patients. These may prove to be effective parameters in the management of the patients with injury.
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    Enzyme linked immunosorbent assay for the diagnosis of human neurocysticercosis: a preliminary report.
    (1990-03-01) Baveja, U K; Arya, G; Goyle, V; Sharma, D K; Gupta, M
    The diagnostic efficacy of an indirect enzyme linked immunosorbent assay (ELISA) was evaluated in cases of neurocystecercosis. Subjects studied included 22 cases and 30 controls (comprising 12 cases of surgically confirmed hydatid disease, 5 cases each of tuberculoma and P.U.O. and 8 cases of pyogenic meningitis). A standard ELISA was performed using porcine cysticerci as antigen. Sensitivity and specificity of the test were calculated for serum and cerebrospinal fluid separately. The test was found to give a sensitivity of 68 and 33 per cent for serum and C.S.F. respectively. The specificity for confirmed case was found to be cent per cent for both with the criteria of reporting used. The sensitivity of the test may be increased by using purified specific antigen and/or sandwich ELISA instead of indirect ELISA technique.
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    Experience in adult population in dengue outbreak in Delhi.
    (1998-03-29) Tripathi, B K; Gupta, B; Sinha, R S; Prasad, S; Sharma, D K
    A dengue outbreak has recently hit the Indian capital. We studied the clinical profile of adult patients. Five hundred and sixty patients of dengue infection were admitted in a specially created ward according to the criteria laid down by WHO. Haematemesis (28.28%), epistaxis (26.78%) and malena (14.28%) were some of the common presentations. Similarly lymphadenopathy, especially cervical (30.89%), palatal rashes (26.96%) and hepatomegaly (23.75%) were the most commonly encountered findings on physical examination. Most of the cases were of dengue fever with haemorrhage and only 2.5% cases were classified under dengue haemorrhagic fever or dengue shock syndrome. The average hospital stay was 3.4 days but only 9.8 hours in the eleven patients who died, suggesting their late arrival in preterminal situation giving little time for resuscitation. Thrombocytopenia was not a feature and only 12.85% patients had platelet count less than 70,000/cmm. Most of the patients who were admitted with thrombocytopenia, showed normalization in their platelet counts in next few days. Serological examination demonstrated evidence of recent dengue infection in 41.17% patients. Few patients required blood or platelet concentrate transfusion. Eleven patients died, three due to DIC, one of intracranial haemorrhage and seven due to massive gastric haemorrhage. Rest of the patients recovered completely. Thus we can conclude that recent outbreak in Delhi was of dengue fever with haemorrhage and mortality was very low in patients who came early to the hospital.
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    Fasciolopsiasis--a persisting problem in eastern U.P.--a case report.
    (2000-01-14) Muralidhar, S; Srivastava, L; Aggarwal, P; Jain, N; Sharma, D K
    Fasciolopsiasis, or infection by the intestinal fluke, Fascilopsis buski, is endemic in the eastern states of our country. While it is by no means a rarity, especially in the rural set up, awareness regarding this common parasitic infestation is still a much-needed entity. The importance of a strong degree of suspicion and early diagnosis cannot be over emphasised, if a successful campaign is to be launched in its control. With this as our central theme, we proceed to report a case of and unsually heavy Fasciolopsis buski infection in our hospital, which had failed to be diagnosed in a semi urban setup in UP.
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    Gall bladder perforation and biliary peritonitis in a young pregnant lady.
    (2001-11-23) Sharma, D K; Panwar, R S; Kumari, K
    A rare event of acute free perforation of gall bladder with biliary peritonitis in a case of calculous cholecystitis in a 28 years old pregnant lady occurring in the absence of the usual factors associated with gall stone disease is reported. The clinical features resembled acute appendicitis and a pre-operative diagnosis could not be made. It is suggested that a thorough attempt should be made to exclude conditions mimicking appendicitis, including those of the biliary system, on finding a normal appendix at emergency appendicectomy without hesitating to convert to full laparotomy if required.
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    Healthcare associated infections in Paediatric Intensive Care Unit of a tertiary care hospital in India: Hospital stay & extra costs.
    (2016-04) Sodhi, Jitender; Satpathy, Sidhartha; Sharma, D K; Lodha, Rakesh; Kapil, Arti; Wadhwa, Nitya; Gupta, Shakti Kumar
    Background & objectives: Healthcare associated infections (HAIs) increase the length of stay in the hospital and consequently costs as reported from studies done in developed countries. The current study was undertaken to evaluate the impact of HAIs on length of stay and costs of health care in children admitted to Paediatric Intensive Care Unit (PICU) of a tertiary care hospital in north India. Methods: This prospective study was done in the seven bedded PICU of a large multi-specialty tertiary care hospital in New Delhi, India. A total of 20 children with HAI (cases) and 35 children without HAI (controls), admitted to the PICU during the study period (January 2012 to June 2012), were matched for gender, age, and average severity of illness score. Each patient’s length of stay was obtained prospectively. Costs of healthcare were estimated according to traditional and time driven activity based costing methods approach. Results: The median extra length of PICU stay for children with HAI (cases), compared with children with no HAI (controls), was seven days (IQR 3-16). The mean total costs of patients with and without HAI were ` 2,04,787 (US$ 3,413) and ` 56,587 (US$ 943), respectively and the mean difference in the total cost between cases and controls was ` 1,48,200 (95% CI 55,716 to 2,40,685, P<0.01). Interpretation & conclusions: This study highlights the effect of HAI on costs for PICU patients, especially costs due to prolongation of hospital stay, and suggests the need to develop effective strategies for prevention of HAI to reduce costs of health care.
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    Hepatopulmonary amoebiasis. Efficacy of various treatment regimens containing dehydroemetine and/or metronidazole.
    (1990-04-01) Jain, N K; Madan, A; Sharma, T N; Sharma, D K; Mandhana, R G
    Thirty three patients with hepatopulmonary amoebiasis were studied. Pulmonary involvement was observed in 55%, pleuro-pulmonary lesions in 30%. Typical chocolate coloured sputum was obtained in 24% and pleural fluid in 21% of patients. Entamoeba histolytica were demonstrable in the sputum in only 6% and in the pleural fluid in 15%. The best therapeutic results were obtained with a combination of dehydroemetine and metronidazole. However, metronidazole was found to be nearly as effective as dehydroemetine, and metronidazole 400 mg and 800 mg were equally effective.
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    Hypophosphatasia.
    (1991-12-01) Agarwal, R P; Sharma, D K; Upadhyay, V K; Goel, S P; Gupta, P; Singh, R
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    Leucocyte alkaline phosphatase activity in the diagnosis of neonatal bacterial infections.
    (1985-01-01) Sharma, D K; Sehgal, S; Sharma, U; Mehrotra, M L; Agrawal, G C
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    Myositis ossificans progressiva.
    (1991-08-01) Agarwal, R P; Verma, S K; Garg, R K; Upadhyaya, V K; Sharma, D K
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    Neurocysticercosis in children.
    (1991-11-01) Puri, V; Sharma, D K; Kumar, S; Choudhury, V; Gupta, R K; Khalil, A
    Twenty seven cases (13 male, 14 female) in the age group of 3-12 years with cerebral cysticercosis were studied and followed up to 3.9 years (mean 1.85 +/- 0.91 years). Seizures (partial 76% and generalized 23.8%) was the feature in 21 patients (77.7%) and raised intracranial tension in 15 (55.5%). Five patients (18.2%) had meningoencephalitis, while 4 (14.8%) had obstructive hydrocephalus due to intraventricular cysts in the 4th ventricle. Twenty two patients received cysticidal drugs (praziquantel or albendazole), while 8 had surgical intervention (CSF diversion, cyst removal, subtemporal decompression or extirpation of the cortical cyst). Mortality was 18.2%. Survivors had epilepsy in 18 patients (81.8%), dementia in 2 (9%), mental subnormality in 6 (27.2%) and hyperkinesia in 12 (54.5%).
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    Pneumomediastinum in association with pulmonary tuberculosis.
    (1996-11-01) Gupta, A; Jain, N; Singh, C; Sharma, D K