Browsing by Author "Fatima, Nusrat"

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    Antihemolytic Activity of Clerodendrum viscosum Vent. is Mediated by its Antioxidant Effect.
    (2013-01) Rahman, Mijanur; Rahaman, Asiqur; Basunia, Mafroz Ahmed; Fatima, Nusrat; Hossain Shahdat
    Aims: The objective of this study was to evaluate the protective effect of polar extract of Clerodendrum viscosum vent. against in vitro hemolysis of human erythrocytes and its association with the antioxidant activity of C. viscosum. Study Design: Extraction of C. viscosum dried root, in vitro antihemolytic activity assay, lipid peroxidation assay, phytochemical analysis, estimation of polyphenols and flavonoids. Place and Duration of Study: Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka, Bangladesh, between January 2011 and May 2011. Methodology: C. viscosum polar extract-pretreated erythrocytes were hemolysed by hypotonia and oxidizing agent, H2O2. The liberated hemoglobin was determined as a measure of hemolysis. Total reducing power and 2,2-diphenyl-1-picrylhydrazyl (DPPH)- free radical scavenging activity of extract were compared with those of vitamin C. Anti- lipid peroxidation activity of C. viscosum polar extract also was determined by exposing rat brain cortex tissue to Fenton's reagent (H2O2+FeSO4)- induced oxidative stress. Then C. viscosum extract was subjected to estimation of total polyphenols and total flavonoids following qualitative phytochemical analysis. Results: C. viscosum polar extract significantly inhibited in vitro hemolysis. Total reducing power and DPPH-radical scavenging activity were higher than those of vitamin C. In cortical tissue homogenate, C. viscosum polar extract significantly reduced (~38%) the levels of lipid peroxides (LPO). Phytochemical analysis revealed the presence of substantial amount of polyphenols, flavonoids and other antioxidant chemicals in the extract. Conclusion: Present investigation demonstrates that antihemolytic activity of C. viscosum polar extract is mediated by its antioxidant effect.
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    Immunoformatics and Molecular Simulation Study Unfold the Immunological Repertoire of Sabia Virus Glycoprotein G1.
    (2014-01) Rahman, Mohammad Mijanur; Nasiri, Mukaddim Ahmed; Fatima, Nusrat
    Sabia virus Glycoprotein G1 (GP1) is a peripheral membrane protein involved in viral adsorption and consequential pathogenic infection. Glycoprotein G1 (GP1) was subjected to immunoformatic and molecular simulation study to predict effective B-cell and T-cell epitopes with knowledge-based exploration of probable immunological responses the epitopes may elicit. The linear and conformational epitopes of GP1 for B-cell were predicted by immunoformatic tools housed in IEDB recourse. Both CD4+ and CD8+ T-cell epitopes were predicted exploiting IEDB recourse tools whereas CD8+ T cell epitopes were severed further by immune response evoking ability of epitope-MHC complex. Contribution of GP1 to innate antiviral response was evolved by interferon (IFN)-gamma inducing and imitation capacity by immunoformatic and docking study respectively. The results of the B-cell epitope analysis suggest the occurrence of potential linear and conformational epitope with cross-reactivity. A range of T-cell epitopes were assumed to be involved in MHC class I and MHC class II molecule dependent antigen presentation. Glycoprotein G1 (GP1) may induce the IFNgamma but its IFN-gamma mimicking ability (confirmed by docking study) that adduces a prompt immune defense. Therefore, inherent immunological repertoire of GP1 epitopes can be taken in to advantage in future immunization regiment development against Sabia virus.
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    Leucas zeylanica (L.) R. Br. protects ethanol and hydrogen peroxide-induced oxidative stress on hepatic tissue of rats.
    (2013-08) Hossain, Shahdat; Rahman, Mijanur; Fatima, Nusrat; Haque, Mozammel; Islam, Jahirul
    The aim of the study was to evaluate the effect of Leucas zeylanica against oxidative stress on hepatic tissue. Oxidative stress was induced by exposing hepatic tissue to ethanol and Fenton’s reagent (H2O2+FeSO4). The effect of oxidative stress on liver also was evaluated by the determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activity and the levels of lipid peroxide (LPO). The antioxidative activity of L. zeylanica was determined by estimating it ability to inhibit the hepatic levels of lipid peroxide (LPO), as indicator of oxidative stress. Concomitantly, the antioxidant phytochemicals such as polyphenols and flavonoids were assessed against pyrogallol and quercetin standards. The ALT and AST activities and the levels of LPO of hepatic tissue were significantly increased by oxidative stress. L. zeylanica pretreatment, however, significantly repressed the oxidative stress on hepatic tissue, as indicated by the decreased activities of ALT and AST enzymes and levels of LPO. Analyses of the phytochemicals revealed that the extract of L. zeylanica contained substantial amounts of polyphenols (74.32 ± 4.6 μg of pyrogallol equivalent/mg) and flavonoids (15.69 ± 2.2 μg quercetin equivalent/mg of extract). Finally, the results of the present study demonstrated the presence of antioxidant phytochemicals, including polyphenols and flavonoids in L. zeylanica and hence-forth conferred protection against ethanol and H2O2-induced oxidative stress on hepatic tissue.
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    Leucas zeylanica (L.) R. Br. protects ethanol and hydrogen peroxide-induced oxidative stress on hepatic tissue of rats.
    (2013) Hossain, Shahdat; Rahman, Mijanur; Fatima, Nusrat; Haque, Mozammel; Islam, Jahirul
    The aim of the study was to evaluate the effect of Leucas zeylanica against oxidative stress on hepatic tissue. Oxidative stress was induced by exposing hepatic tissue to ethanol and Fenton’s reagent (H2O2+FeSO4). The effect of oxidative stress on liver also was evaluated by the determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activity and the levels of lipid peroxide (LPO). The antioxidative activity of L. zeylanica was determined by estimating it ability to inhibit the hepatic levels of lipid peroxide (LPO), as indicator of oxidative stress. Concomitantly, the antioxidant phytochemicals such as polyphenols and flavonoids were assessed against pyrogallol and quercetin standards. The ALT and AST activities and the levels of LPO of hepatic tissue were significantly increased by oxidative stress. L. zeylanica pretreatment, however, significantly repressed the oxidative stress on hepatic tissue, as indicated by the decreased activities of ALT and AST enzymes and levels of LPO. Analyses of the phytochemicals revealed that the extract of L. zeylanica contained substantial amounts of polyphenols (74.32 ± 4.6 μg of pyrogallol equivalent/mg) and flavonoids (15.69 ± 2.2 μg quercetin equivalent/mg of extract). Finally, the results of the present study demonstrated the presence of antioxidant phytochemicals, including polyphenols and flavonoids in L. zeylanica and hence-forth conferred protection against ethanol and H2O2-induced oxidative stress on hepatic tissue.