Enantioselective hydrolysis of epichlorohydrin using whole Aspergillus niger ZJB-09173 cells in organic solvents.

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dc.contributor.authorJin, Huo-Xi
dc.contributor.authorHu, Zhong-Ce
dc.contributor.authorZheng, Yu-Guo
dc.date.accessioned2015-08-11T03:39:29Z
dc.date.available2015-08-11T03:39:29Z
dc.date.issued2012-09
dc.description.abstractThe enantioselective hydrolysis of racemic epichlorohydrin for the production of enantiopure (S)-epichlorohydrin using whole cells of Aspergillus niger ZJB-09173 in organic solvents was investigated. Cyclohexane was used as the reaction medium based on the excellent enantioselectivity of epoxide hydrolase from A. niger ZJB- 09173 in cyclohexane. However, cyclohexane had a negative effect on the stability of epoxide hydrolase from A. niger ZJB-09173. In the cyclohexane medium, substrate inhibition, rather than product inhibition of catalysis, was observed in the hydrolysis of racemic epichlorohydrin using A. niger ZJB-09173. The racemic epichlorohydrin concentration was markedly increased by continuous feeding of substrate without significant decline of the yield. Ultimately, 18.5% of (S)-epichlorohydrin with 98% enantiomeric excess from 153.6 mM of racemic epichlorohydrin was obtained by the dry cells of A. niger ZJB-09173, which was the highest substrate concentration in the production of enantiopure (S)-epichlorohydrin by epoxide hydrolases using an organic solvent medium among the known reports.en_US
dc.identifier.citationJin Huo-Xi, Hu Zhong-Ce, Zheng Yu-Guo. Enantioselective hydrolysis of epichlorohydrin using whole Aspergillus niger ZJB-09173 cells in organic solvents. Journal of Biosciences. 2012 Sep; 37 (4): 695-702.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/161732
dc.language.isoenen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/sep2012/695.pdfen_US
dc.subjectEnantioselective hydrolysisen_US
dc.subjectenantioselectivityen_US
dc.subjectepichlorohydrinen_US
dc.subjectepoxide hydrolaseen_US
dc.subjectorganic solventen_US
dc.titleEnantioselective hydrolysis of epichlorohydrin using whole Aspergillus niger ZJB-09173 cells in organic solvents.en_US
dc.typeArticleen_US
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