In vitro regeneration and ploidy level analysis of Eulophia ochreata Lindl.

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dc.contributor.authorShriram, Varsha
dc.contributor.authorNanekar, Vikas
dc.contributor.authorKumar, Vinay
dc.contributor.authorKishor, P B Kavi
dc.date.accessioned2014-12-12T11:33:11Z
dc.date.available2014-12-12T11:33:11Z
dc.date.issued2014-11
dc.description.abstractVarious parameters including explant-type, medium compositions, use of phytohormones and additives were optimized for direct and indirect regeneration of E. ochreata, a medicinal orchid under threat. Protocorm-like-bodies (PLBs) proved to be the best explants for shoot initiation, proliferation and callus induction. Murashige and Skoog’s (MS) medium containing 2.5 mg L-1 6-benzylaminopurine (BAP), 1.0 mg L-1 kinetin (Kin) and additives (adenine sulfate, arginine, citric acid, 30 mg L-1 each and 50 mg L-1 ascorbic acid) was optimal for shoot multiplication (12.1 shoots and 7.1 PLBs per explant with synchronized growth), which also produced callus. Shoot number was further increased with three successive subcultures on same media and ~40 shoots per explant were achieved after 3 cycles of 30 days each. Additives and casein hydrolysate (CH) showed advantageous effects on indirect shoot regeneration via protocorm-derived callus. Optimum indirect regeneration was achieved on MS containing additives, 500 mg L-1 CH, 2.5 mg L-1 BAP and 1.0 mg L-1 Kin with 30 PLBs and 6 shoots per callus mass (~5 mm size). The shoots were rooted (70% frequency) on one by fourth-MS medium containing 2.0 mg L-1 indole-3-butyric acid, 200 mg L-1 activated charcoal and additives. The rooted plantlets were hardened and transferred to greenhouse with 63% survival rate. Flow-cytometry based DNA content analysis revealed that the ploidy levels were maintained in in vitro regenerated plants. This is the first report for in vitro plant regeneration in E. ochreata.en_US
dc.identifier.citationShriram Varsha, Nanekar Vikas, Kumar Vinay, Kishor P B Kavi. In vitro regeneration and ploidy level analysis of Eulophia ochreata Lindl. Indian Journal of Experimental Biology. 2014 Nov; 52(11): 1112-1121.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153800
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29753en_US
dc.subjectDNA ploidyen_US
dc.subjectEulophiaen_US
dc.subjectFlow cytometryen_US
dc.subjectMultiple shootsen_US
dc.subjectOrchidsen_US
dc.subjectProtocorm-like-bodiesen_US
dc.subject.meshAscorbic Acid --pharmacology
dc.subject.meshCaesins --pharmacology
dc.subject.meshChromosomes, Plant
dc.subject.meshCitric Acid --pharmacology
dc.subject.meshCulture Media --pharmacology
dc.subject.meshCytokinins --pharmacology
dc.subject.meshIndoleacetic Acid --pharmacology
dc.subject.meshOrchidaceae --genetics
dc.subject.meshOrchidaceae --growth & development
dc.subject.meshOrchidaceae --physiology
dc.subject.meshOrganoids --drug effects
dc.subject.meshOrganoids --physiology
dc.subject.meshPlant Cells --drug effects
dc.subject.meshPlant Cells --physiology
dc.subject.meshPlant Leaves --drug effects
dc.subject.meshPlant Leaves --growth & development
dc.subject.meshPlant Shoots --drug effects
dc.subject.meshPlant Shoots --growth & development
dc.subject.meshPlants, Medicinal --genetics
dc.subject.meshPlants, Medicinal --growth & development
dc.subject.meshPlants, Medicinal --physiology
dc.subject.meshPloidies
dc.subject.meshRegeneration
dc.subject.meshRhizomes --drug effects
dc.subject.meshRhizomes --growth & development
dc.subject.otherTissue Culture Techniques
dc.titleIn vitro regeneration and ploidy level analysis of Eulophia ochreata Lindl.en_US
dc.typeArticleen_US
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