Development of an enzyme immunoassay using recombinant invariant surface glycoprotein (rISG) 75 for serodiagnosis of bovine trypanosomosis.

Simple item page
dc.contributor.authorRudramurthy, G R
dc.contributor.authorSengupta, P P
dc.contributor.authorMetilda, B
dc.contributor.authorBalamurugan, V
dc.contributor.authorPrabhudas, K
dc.contributor.authorRahman, H
dc.date.accessioned2015-01-10T10:55:45Z
dc.date.available2015-01-10T10:55:45Z
dc.date.issued2015-01
dc.description.abstractTrypanosomosis or surra is caused by the haemoflagellate parasite, Trypanosoma evansi and is an important disease of animals, including domestic and wild herbivores and carnivores, in tropical countries. The invariant surface glycoproteins (ISGs) are blood stream stage specific and are uniformly distributed over the entire surface of the trypanosomes. In the present study, the extracellular domain (ED) region of ISG-75 from T. evansi, consisting of 1320 nt, encoding a polypeptide of 440 amino acids, has been heterologously expressed in Escherichia coli. Further, the immunoreactivity of recombinant ISG-75 (rISG-75) was characterized in immunoblot and ELISA using T. evansi hyper immune sera raised in experimental animals. The protein was found immunoreactive when compared with a panel of antigens (VSG RoTat 1.2 and whole cell lysate) using bovine serum samples from field. The diagnostic potential of rISG-75 was evaluated in ELISA with large number of bovine field serum samples. The optimum sensitivity and specificity were 98.47 and 99.1, respectively. The present finding showed that the expressed protein has potential use in the serodiagnosis of trypanosomosis.en_US
dc.identifier.citationRudramurthy G R, Sengupta P P, Metilda B, Balamurugan V, Prabhudas K, Rahman H. Development of an enzyme immunoassay using recombinant invariant surface glycoprotein (rISG) 75 for serodiagnosis of bovine trypanosomosis. Indian Journal of Experimental Biology. 2015 Jan; 53(1): 7-15.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/154985
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/30275en_US
dc.subjectCattleen_US
dc.subjectExtracellular domainen_US
dc.subjectParasitaemiaen_US
dc.subjectSurraen_US
dc.subjectTrypanosoma evansien_US
dc.subject.meshAnimals
dc.subject.meshBase Sequence
dc.subject.meshCattle
dc.subject.meshCattle Diseases --diagnosis
dc.subject.meshDNA Primers
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methods
dc.subject.meshMembrane Glycoproteins --diagnostic use
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshProtozoan Proteins --diagnostic use
dc.subject.meshRecombinant Proteins --diagnostic use
dc.subject.meshSensitivity and Specificity
dc.subject.meshSerologic Tests --methods
dc.subject.meshTrypanosomiasis --diagnosis
dc.subject.meshTrypanosomiasis --veterinary
dc.titleDevelopment of an enzyme immunoassay using recombinant invariant surface glycoprotein (rISG) 75 for serodiagnosis of bovine trypanosomosis.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2015v53n1p7.pdf
Size:
300.85 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology