Investigation of Ureaplasma urealyticum biovars and their relationship with antimicrobial resistance.

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dc.contributor.authorChang-tai, Zhu
dc.contributor.authorZhong-yi, Hu
dc.contributor.authorChun-lei, Dong
dc.contributor.authorChang-song, Zhang
dc.contributor.authorMei-zhen, Wan
dc.contributor.authorYang, Ling
dc.date.accessioned2012-12-14T04:06:30Z
dc.date.available2012-12-14T04:06:30Z
dc.date.issued2011-07
dc.description.abstractPurpose: To develop Taqman fluorescence quantitative polymerase chain reaction (PCR) method for investigating the characteristics of the distributions of Ureaplasma urealyticum (UU) biovars and to explore the relationship between UU biovars and antimicrobial resistance. Materials and Methods: By the method of culture, Ureaplasma species were detected. Taqman fluorescence quantitative PCR for detecting UU biovars were developed and UU clinical isolates were detected to distinguish biovars. The broth micro-dilution susceptibility testing methods were used to determine UU susceptibility. Results: By Taqman PCR method, UU biovars was successfully detected. Of 126 samples, biovar 1 was found in 73 (57.94%). There was a statistical difference between genital-urinary tract infection group and asymptomatic group (P<0.05). In the region, UU biovar 1 to 9 kinds of agents kept higher susceptibility rates, but biovar 2 maintained higher susceptibility rates only to tetracyclines. Compared with biovar 1, UU biovar 2 resistance rates to 7 kinds of agents were higher (P<0.05). Conclusions: (1) Our new established Taqman PCR method is a useful tool for screening UU biovars. (2) UU biovar 1 predominated in asymptomatic population; whereas in genital-urinary tract infection population UU biovar 2 had a higher proportion. (3) The characteristics of drug resistance were different between UU biovars. Overall, both two biovars remained higher susceptibility rates to tetracyclines. A majority of biovor 1 strains were sensitive to macrolides and quinolones; while only a small number of biovar 2 strains kept sensitive to roxithromycin and quinolones, a large proportion of biovar 2 strains were found in intermediate ranges.en_US
dc.identifier.citationChang-tai Zhu, Zhong-yi Hu, Chun-lei Dong, Chang-song Zhang, Mei-zhen Wan, Yang Ling. Investigation of Ureaplasma urealyticum biovars and their relationship with antimicrobial resistance. Indian Journal of Medical Microbiology. 2011 Jul-Sept; 29(3): 288-292.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143836
dc.language.isoenen_US
dc.source.urihttps://www.ijmm.org/article.asp?issn=0255-0857;year=2011;volume=29;issue=3;spage=288;epage=292;aulast=Chang-taien_US
dc.subjectAntimicrobial susceptibilityen_US
dc.subjectbiovaren_US
dc.subjectTaqman fluorescence quantitative PCRen_US
dc.subjectUreaplasma urealyticumen_US
dc.subject.meshBacterial Typing Techniques
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshPolymerase Chain Reaction --methods
dc.subject.meshUreaplasma urealyticum --classification
dc.subject.meshUreaplasma urealyticum --drug effects
dc.subject.meshUreaplasma urealyticum --genetics
dc.titleInvestigation of Ureaplasma urealyticum biovars and their relationship with antimicrobial resistance.en_US
dc.typeArticleen_US
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