Isolation of a haemorrhagic protein toxin (SA-HT) from the Indian venomous butterfish (Scatophagus argus, Linn) sting extract.

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dc.contributor.authorKarmakar, Sen_US
dc.contributor.authorMuhuri, D Cen_US
dc.contributor.authorDasgupta, S Cen_US
dc.contributor.authorNagchaudhuri, A Ken_US
dc.contributor.authorGomes, Aen_US
dc.date.accessioned2009-05-28T10:42:53Z
dc.date.available2009-05-28T10:42:53Z
dc.date.issued2004-05-06en_US
dc.description.abstractA haemorrhagic protein toxin (SA-HT) was isolated and purified from the spine extract of the Indian venomous butterfish, S. argus Linn, by two step ion exchange chromatography. The toxin was homogeneous in native and SDS-PAGE gel. SDS-molecular weight of the toxin was found to be 18.1 +/- 0.09 kDa. SA-HT produced severe haemorrhage on stomach wall but devoid of cutaneous haemorrhage. UV, EDTA, trypsin, protease, cyproheptadine, indomethacin, acetylsalicylic acid and BW755C treatment significantly antagonized the haemorrhagic activity of SA-HT. The toxin produced dose and time dependent oedema on mice hind paw, which was significantly encountered by cyproheptadine, indomethacin and BW755C. SA-HT increased capillary permeability on guinea pig dorsal flank. On isolated guineapig ileum, rat fundus and uterus, SA-HT produced slow contraction which was completely antagonised by prostaglandin blocker SC19220. On isolated rat duodenum, SA-HT produced slow relaxation. SA-HT significantly increased plasma plasmin, serum MDA level and decreased serum SOD level indicating the possible involvement of cyclooxygenase and lipooxygenase pathway.en_US
dc.description.affiliationPostgraduate Department of Zoology, Maulana Azad College, Kolkata 700 013, India.en_US
dc.identifier.citationKarmakar S, Muhuri DC, Dasgupta SC, Nagchaudhuri AK, Gomes A. Isolation of a haemorrhagic protein toxin (SA-HT) from the Indian venomous butterfish (Scatophagus argus, Linn) sting extract. Indian Journal of Experimental Biology. 2004 May; 42(5): 452-60en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/55694
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.mesh4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine --pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnti-Inflammatory Agents, Non-Steroidal --pharmacologyen_US
dc.subject.meshAspirin --pharmacologyen_US
dc.subject.meshCapillariesen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshCyproheptadine --pharmacologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshEdema --chemically induceden_US
dc.subject.meshEdetic Acid --pharmacologyen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshFemaleen_US
dc.subject.meshFish Proteins --chemistryen_US
dc.subject.meshFish Venoms --chemistryen_US
dc.subject.meshGastrointestinal Agents --pharmacologyen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshIndomethacin --pharmacologyen_US
dc.subject.meshLipoxygenase --metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMuscle, Smooth --drug effectsen_US
dc.subject.meshPerciformesen_US
dc.subject.meshPermeabilityen_US
dc.subject.meshRatsen_US
dc.subject.meshSpine --metabolismen_US
dc.subject.meshSuperoxide Dismutase --metabolismen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTrypsin --pharmacologyen_US
dc.subject.meshUltraviolet Raysen_US
dc.subject.meshUterus --drug effectsen_US
dc.titleIsolation of a haemorrhagic protein toxin (SA-HT) from the Indian venomous butterfish (Scatophagus argus, Linn) sting extract.en_US
dc.typeJournal Articleen_US
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