Exploration of new effective fibrinolytic agent from Alcaligenes aquatilis PJS_1 from polluted soil

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dc.contributor.authorN., Prabhuen_US
dc.contributor.authorK., Sangeethaen_US
dc.contributor.authorC., Jayanthien_US
dc.contributor.authorT., Gajendranen_US
dc.date.accessioned2020-11-18T10:16:10Z
dc.date.available2020-11-18T10:16:10Z
dc.date.issued2020-05
dc.description.abstractAim: To isolate and identify Alcaligenes aquatilis PJS_1 from slaughter house soil samples for production of enzymatic fibrinolytic agent productionMethodology: Fibrinolytic enzyme producing bacterium was isolated from slaughter house soil samples and identified by biochemical tests and 16S rRNA sequencing. The fibrinolytic enzyme production media was optimized by various factors like energy sources, pH and temperature. Bioreactor used in the experiment was designed with suitable parameters for effective production and purification is by gel filtration chromatography. Blood clotting assay was performed to determine its anticoagulant property. Results: The isolated enzyme producing bacterium was identified as Alcaligenes aquatilis PJS_1. The medium with fructose and urea at pH 7.0 was found to have optimum production when incubated for 24 hr at 37ÂșC. The crude enzyme was purified by acetone precipitation followed by gel filtration chromatography. The enzyme showed a final specific activity of 629.32 Umg-1 with of 88.24% yield Interpretation: The present study provides information that the enzyme produced by Alcaligenes aquatilis PJS_1 acts as an effective fibrinolytic agenten_US
dc.identifier.affiliationsDepartment of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637 205, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637 205, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637 205, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli-620 024, Indiaen_US
dc.identifier.citationN. Prabhu, K. Sangeetha, C. Jayanthi, T. Gajendran. Exploration of new effective fibrinolytic agent from Alcaligenes aquatilis PJS_1 from polluted soil. Journal of Environmental Biology . 2020 May; 41(3): 572-580en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214512
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber3en_US
dc.relation.volume41en_US
dc.source.urihttps://www.jeb.co.in/journal_issues/202005_may20/paper_07.pdfen_US
dc.subjectAlcaligenes sp.en_US
dc.subjectAnticoagulanten_US
dc.subjectBioreactoren_US
dc.subjectFibrinolytic enzymeen_US
dc.titleExploration of new effective fibrinolytic agent from Alcaligenes aquatilis PJS_1 from polluted soilen_US
dc.typeJournal Articleen_US
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