Cultivation of buffalo green monkey kidney cells persistently infected with hepatitis A virus.

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dc.contributor.authorChitambar, S Den_US
dc.contributor.authorGrewal, S Men_US
dc.contributor.authorBokil, Men_US
dc.contributor.authorSrinivasan, M Aen_US
dc.contributor.authorBanerjee, Ken_US
dc.date.accessioned1994-03-01en_US
dc.date.accessioned2009-05-27T07:30:00Z
dc.date.available1994-03-01en_US
dc.date.available2009-05-27T07:30:00Z
dc.date.issued1994-03-01en_US
dc.description.abstractStudies were carried out to determine the effect of prolongation of incubation periods, cocultivation with normal buffalo green monkey kidney (BGMK) cells and different concentrations of foetal calf serum (FCS) on the production of hepatitis A virus (HAV) by BGMK cell line persistently infected with HAV strain HM175. HAV could be detected from week 1 onwards. However, maintenance of cultures beyond this period was found to yield substantially higher quantities of virus. Cocultivation of persistently infected cells with normal BGMK cells also improved the antigen yields. Different concentrations of FCS did not show any effect on the amount of virus produced. The cell line was maintained up to 46 passages during which there was continuous production of HAV in the cells and release of small amounts of virus in the culture supernatants. Cell associated and cell free viral particles were found to be infectious. Supernatant derived virus was a highly suitable inoculum for infecting other susceptible cell lines. Persistently infected BGMK cell line appears to be a reliable and economical source to derive HAV in adequate amounts for diagnostic and research purposes.en_US
dc.description.affiliationNational Institute of Virology, Pune.en_US
dc.identifier.citationChitambar SD, Grewal SM, Bokil M, Srinivasan MA, Banerjee K. Cultivation of buffalo green monkey kidney cells persistently infected with hepatitis A virus. Indian Journal of Medical Research. 1994 Mar; 99(): 115-20en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/20955
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Line --microbiologyen_US
dc.subject.meshCercopithecus aethiops --microbiologyen_US
dc.subject.meshChronic Diseaseen_US
dc.subject.meshHepatitis A --diagnosisen_US
dc.subject.meshHepatovirus --growth & developmenten_US
dc.subject.meshKidney --microbiologyen_US
dc.subject.meshVirus Cultivation --methodsen_US
dc.titleCultivation of buffalo green monkey kidney cells persistently infected with hepatitis A virus.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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