Immunoblot analysis of antibody responses to Shigella dysenteriae type 1 infection.

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dc.contributor.authorSinha, A Ken_US
dc.contributor.authorChakraborti, Sen_US
dc.date.accessioned1992-05-01en_US
dc.date.accessioned2009-05-27T06:33:56Z
dc.date.available1992-05-01en_US
dc.date.available2009-05-27T06:33:56Z
dc.date.issued1992-05-01en_US
dc.description.abstractImmunoblot analysis was used to identify the antigenic components of the outer membrane protein (OMP) extract from Sh. dysenteriae type 1 that may be relevant in protection against infection. The OMPs were extracted by ultrasonic disruption followed by Sarkosyl extraction. The macromolecular bands were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), electroeluted to a nitrocellulose matrix and complexed with peroxidase conjugated antibodies in human convalescent sera. IgG specific reaction was found to a major antigenic component at 57 kilodaltons. In addition, weakly reactive three antigenic determinants of greater than 57 kD and four of less than 57 kD were found. Control sera from a rabbit immunized against OMP also exhibited a similar pattern of antigenic reactivity. Some of the OMPs with high antigenicity may be important in immunoprophylaxis.en_US
dc.description.affiliationNational Institute of Cholera & Enteric Diseases, Calcutta.en_US
dc.identifier.citationSinha AK, Chakraborti S. Immunoblot analysis of antibody responses to Shigella dysenteriae type 1 infection. Indian Journal of Medical Research. 1992 May; 95(): 112-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/16861
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAntibodies, Bacterial --blooden_US
dc.subject.meshBacterial Outer Membrane Proteins --immunologyen_US
dc.subject.meshDysentery, Bacillary --immunologyen_US
dc.subject.meshElectrophoresisen_US
dc.subject.meshEpitopes --immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshShigella dysenteriae --immunologyen_US
dc.titleImmunoblot analysis of antibody responses to Shigella dysenteriae type 1 infection.en_US
dc.typeJournal Articleen_US
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