Detection of GSTM1 and GSTT1 Polymorphisms in Nasopharyngeal Carcinoma Patients by Multiplex PCR Assay
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Date
2011-02-03
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Thai Cancer Journal
Abstract
Nasopharyngeal carcinoma (NPC) is a common cancer and a serious public-health problem in Thailand. Glutathione S-transferase Mu1 and Theta1 gene (GSTM1 and GSTT1) are involved in the prevention of cancer, by encoding GSTM1 and GSTT1 enzymes to detoxify various electrophiles derived from environmental carcinogens. GSTM1 and GSTT1 polymorphisms are reportedly associated with several malignancies and can be used as a genetic risk marker for cancer. Nevertheless, GSTM1 and GSTT1 polymorphism detection using the conventional polymerase chain reaction (C-PCR) assay is complicated and time-consuming, and thus unsuitable for mass screening. A faster multiplex PCR (M-PCR) assay has been developed to help overcome these problems. The present study aimed to establish the M-PCR assay for GSTM1 and GSTT1 polymorphism detection in NPC patients, and confirm the results of the new assay with the C-PCR assay. Eighty DNA samples, extracted from the peripheral blood leukocytes of Thai NPC patients, were examined for GSTM1 and GSTT1 polymorphism [GSTM1 normal genotype (GSTM1+), GSTM1 null genotype (GSTM1-), GSTT1 normal genotype (GSTT1+), and GSTT1 null genotype (GSTT1-)] by M-PCR and C-PCR assays. The GSTM1 and GSTT1 polymorphism-detection results in all NPC cases by M-PCR assay agreed with the C-PCR assay ( = 1.0). In addition, the M-PCR assay was a simple, faster, and less costly method for GSTM1 and GSTT1 polymorphism detection than the C-PCR assay. The present study suggests that GSTM1 and GSTT1 polymorphism detection by M-PCR assay is a reliable and suitable tool for screening high-risk NPC groups. (Thai Cancer J 2010;30:94-103)
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Thai Cancer Journal; Vol.30 No.3 July-September 2010; 94-103