Reverse single radial immunodiffusion for estimation of titre of anti IgG antibody.

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dc.contributor.authorThakar, Y Sen_US
dc.contributor.authorKulkarni, Cen_US
dc.contributor.authorPande, Sen_US
dc.contributor.authorDhanvijay, A Gen_US
dc.contributor.authorShrikhande, A Ven_US
dc.contributor.authorSaoji, A Men_US
dc.date.accessioned2009-05-28T15:18:19Z
dc.date.available2009-05-28T15:18:19Z
dc.date.issued1993-05-01en_US
dc.description.abstractReverse Single Radial Immunodiffusion (SRID) for estimating titre of anti IgG antisera is reported. Unlike the conventional radial immunodiffusion, the antigen (IgG) is held immobile in the gel while the antibody (Anti IgG) diffuses radially from the well (7 microliters) and the diameter of the resulting immuneprecipitates after immunodiffusion at 4 degrees C for 24 hr, represents a linear correlation with the antibody titre. The procedure was standardised by an extensive trial and error employing different concentrations of human IgG in the gel (60-240 micrograms) against varying dilutions of the standard antibody (titre: 3.8 mg/ml). The best results were obtained at 80 micrograms of IgG in the gel. The locally raised rabbit anti IgG antisera displayed a distinctive titre pattern under optimised conditions. Technical reproducibility, high-sensitivity threshold (0.25 mg/ml), simultaneous visual scrutiny of several antibody batches at a glance and ability to assess the shelf life of the stored antisera are its distinct assets.en_US
dc.description.affiliationDepartment of Microbiology, Government Medical College, Nagpur, India.en_US
dc.identifier.citationThakar YS, Kulkarni C, Pande S, Dhanvijay AG, Shrikhande AV, Saoji AM. Reverse single radial immunodiffusion for estimation of titre of anti IgG antibody. Indian Journal of Experimental Biology. 1993 May; 31(5): 426-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/61815
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAntibodies, Anti-Idiotypic --analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunodiffusion --methodsen_US
dc.subject.meshImmunoglobulin G --immunologyen_US
dc.titleReverse single radial immunodiffusion for estimation of titre of anti IgG antibody.en_US
dc.typeJournal Articleen_US
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