Beta-fructofuranosidase production by 2-deoxyglucose resistant mutants of aspergillus niger in submerged and solid-state fermentation.

Simple item page
dc.contributor.authorAshokkumar, Balasubramaniemen_US
dc.contributor.authorGunasekaran, Paramasamyen_US
dc.date.accessioned2009-05-28T11:16:35Z
dc.date.available2009-05-28T11:16:35Z
dc.date.issued2002-09-18en_US
dc.description.abstractAspergillus niger produces extracellular beta-fructofuranosidase under submerged (SmF) and solid state fermentation (SSF) conditions. After UV mutagenesis of conidiospores of A. niger, 2-deoxyglucose (10 g/l) resistant mutants were isolated on Czapek's minimal medium containing glycerol as a carbon source and the mutants were examined for improved production of beta-fructofuranosidase in SmF and SSF conditions. One of such mutant DGRA-1 overproduced beta-fructofuranosidase in both SmF and SSF conditions. In SmF, the mutant DGRA-1 showed higher beta-fructofuranosidase productivity (110.8 U/l/hr) than the wild type (48.3 U/l/hr). While in SSF the same strain produced 322 U/l/hr of beta-fructofuranosidase, 2 times higher than that of wild type (154.2 U/l/hr). In SmF, both wild type and mutants produced relatively low level of beta-fructofuranosidase in medium containing sucrose with glucose than from the sucrose medium. However in SSF, the DGRA-1 mutant grown in sucrose and sucrose+ glucose did not show any difference with respect to beta-fructofuranosidase production. These results indicate that the catabolite repression of beta-fructofuranosidase synthesis is observed in SmF whereas in SSF such regulation was not prominent.en_US
dc.description.affiliationCentre for Advanced Studies in Functional Genomics, School of Biological Sciences, Madurai Kamaraj University, Madurai 625 021, India.en_US
dc.identifier.citationAshokkumar B, Gunasekaran P. Beta-fructofuranosidase production by 2-deoxyglucose resistant mutants of aspergillus niger in submerged and solid-state fermentation. Indian Journal of Experimental Biology. 2002 Sep; 40(9): 1032-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56488
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAspergillus niger --drug effectsen_US
dc.subject.meshDeoxyglucose --pharmacologyen_US
dc.subject.meshDrug Resistanceen_US
dc.subject.meshDrug Resistance, Fungalen_US
dc.subject.meshFermentationen_US
dc.subject.meshGlucose --metabolismen_US
dc.subject.meshGlycoside Hydrolases --biosynthesisen_US
dc.subject.meshIndustrial Microbiologyen_US
dc.subject.meshMutagenesis, Site-Directeden_US
dc.subject.meshMutationen_US
dc.subject.meshSucrose --metabolismen_US
dc.subject.meshUltraviolet Raysen_US
dc.subject.meshbeta-Fructofuranosidaseen_US
dc.titleBeta-fructofuranosidase production by 2-deoxyglucose resistant mutants of aspergillus niger in submerged and solid-state fermentation.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Experimental Biology