Establishing gene Amelogenin as sex-specific marker in yak by genomic approach

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dc.contributor.authorDas, P. P.en_US
dc.contributor.authorKRISHNAN, G.en_US
dc.contributor.authorDOLEY, J.en_US
dc.contributor.authorBHATTACHARYA, D.en_US
dc.contributor.authorDEB, S. M.en_US
dc.contributor.authorCHAKRAVARTY, P.en_US
dc.contributor.authorDAS, P. J.en_US
dc.date.accessioned2020-11-18T10:20:45Z
dc.date.available2020-11-18T10:20:45Z
dc.date.issued2019-02
dc.description.abstractYak, an economically important bovine species considered as lifeline of the Himalaya. Indeed, this gigantic bovine is neglected because of the scientific intervention for its conservation as well as research documentation for a long time. Amelogenin is an essential protein for tooth enamel which eutherian mammals contain two copies in both X and Y chromosome each. In bovine, the deletion of a fragment of the nucleotide sequence in Y chromosome copy of exon 6 made Amelogenin an excellent sex-specific marker. Thus, an attempt was made to use the gene as an advanced molecular marker of sexing of the yak to improve breeding strategies and reproduction. The present study confirmed that the polymerase chain reaction amplification of the Amelogenin gene with a unique primer is useful in sex identification of the yak. The test is further refined with qPCR validation by quantifying the DNA copy number of the Amelogenin gene in male and female. We observed a high level of sequence polymorphisms of AMELX and AMELY in yak considered as novel identification. These tests can be further extended into several other specialized fields includingforensics, meat production and processing, and quality control.en_US
dc.identifier.affiliationsIndian Council of Agricultural Research-National Research Centre on Yak, Dirang 790 101, Indiaen_US
dc.identifier.affiliationsIndian Council of Agricultural Research -National Institute of Animal Nutrition and Physiology, Bengaluru 560 030, Indiaen_US
dc.identifier.affiliationsIndian Council of Agricultural Research -Central Island Agricultural Research Institute, Garacharama, Port Blair 744 101, Indiaen_US
dc.identifier.affiliationsIndian Council of Agricultural Research -National Dairy Research Institute, Karnal 132 001, Indiaen_US
dc.identifier.affiliationsIndian Council of Agricultural Research-National Research Centre on Yak, Dirang 790 101, Indiaen_US
dc.identifier.affiliationsIndian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati 781 131, Indiaen_US
dc.identifier.citationDas P. P., Krishnan G., Doley J., Bhattacharya D., Deb S. M., Chakravarty P., Das P. J.. Establishing gene Amelogenin as sex-specific marker in yak by genomic approach. Journal of Genetics. 2019 Feb; 98: 1-6en_US
dc.identifier.issn0022-1333
dc.identifier.issn0973-7731
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215379
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume98en_US
dc.source.urihttps://www.ias.ac.in/article/fulltext/jgen/098/0007en_US
dc.subjectyaken_US
dc.subjectY-chromosomeen_US
dc.subjectAMELX geneen_US
dc.subjectAMELY geneen_US
dc.subjectmarkeren_US
dc.subjectsex determination.en_US
dc.titleEstablishing gene Amelogenin as sex-specific marker in yak by genomic approachen_US
dc.typeJournal Articleen_US
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