Application of pulsed field gel electrophoresis for molecular characterization of vancomycin resistant Staphylococcus aureus isolates from hospitals

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dc.contributor.authorTalwar, A.en_US
dc.contributor.authorKumar, S.en_US
dc.date.accessioned2020-11-18T10:16:09Z
dc.date.available2020-11-18T10:16:09Z
dc.date.issued2020-03
dc.description.abstractAim: The present study was conducted to characterize VRSA isolates on the basis of pulsed field gel electrophoresis (PFGE) and the presence of spa gene, recovered from different Doon Valley Hospitals.Methodology: Six VRSA isolates were analyzed using PFGE and spa typing. spa gene coded Protein A was used as a genetic marker for the characterization of Staphylococcus aureus isolates. Dendrogram were constructed on the basis of unweighted pair group method with arithmetic means (UPGMA method) for clusters analyses.Results: Dendrogram finally showed two major banding patterns at about 85% similarity designated as PFGE type A and PFGE type B exhibiting differences of 4-6 bands. The length of spa gene varied from 1200 to 1500 bp, showing variation in length. The most prevalent length was 1200bp.en_US
dc.identifier.affiliationsDepartment of Microbiology, Doon (PG) Paramedical College and Hospital, Dehradun-248 001, Indiaen_US
dc.identifier.affiliationsInstitute of Pharma Sciences, Chandigarh University, Chandigarh-160 036, Indiaen_US
dc.identifier.citationTalwar A., Kumar S.. Application of pulsed field gel electrophoresis for molecular characterization of vancomycin resistant Staphylococcus aureus isolates from hospitals. Journal of Environmental Biology . 2020 Mar; 41(2): 202-206en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214494
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber2en_US
dc.relation.volume41en_US
dc.source.urihttps://www.jeb.co.in/journal_issues/202003_mar20/paper_08.pdfen_US
dc.subjectPulse field gel electrophoresisen_US
dc.subjectSpa geneen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectVancomycin resistanten_US
dc.subjectVRSA clonesen_US
dc.titleApplication of pulsed field gel electrophoresis for molecular characterization of vancomycin resistant Staphylococcus aureus isolates from hospitalsen_US
dc.typeJournal Articleen_US
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