Potential application of β-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction.

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dc.contributor.authorJose, Divya
dc.contributor.authorJayesh, P
dc.contributor.authorGopinath, Prem
dc.contributor.authorMohandas, A
dc.contributor.authorSingh, I S Bright
dc.date.accessioned2014-04-29T10:24:31Z
dc.date.available2014-04-29T10:24:31Z
dc.date.issued2014-01
dc.description.abstractPseudomonas aeruginosa MCCB 123 was grown in a synthetic medium for β-1,3 glucanase production. From the culture filtrate, β-1,3 glucanase was purified with a molecular mass of 45 kDa. The enzyme was a metallozyme as its β-1,3 glucanase activity got inhibited by the metal chelator EDTA. Optimum pH and temperature for β-1,3 glucanase activity on laminarin was found to be 7 and 50 °C respectively. The MCCB 123 β-1,3 glucanase was found to have good lytic action on a wide range of fungal isolates, and hence its application in fungal DNA extraction was evaluated. β-1,3 glucanase purified from the culture supernatant of P. aeruginosa MCCB 123 could be used for the extraction of fungal DNA without the addition of any other reagents generally used. Optimum pH and temperature of enzyme for fungal DNA extraction was found to be 7 and 65 °C respectively. This is the first report on β-1,3 glucanase employed in fungal DNA extraction.en_US
dc.identifier.citationJose Divya, Jayesh P, Gopinath Prem, Mohandas A, Singh I S Bright. Potential application of β-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction. Indian Journal of Experimental Biology. 2014 Jan; 52(1): 89-96.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/150337
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/25148en_US
dc.subjectβ-1,3 glucanaseen_US
dc.subjectDNA extractionen_US
dc.subjectFungusen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subject.meshDNA, Fungal --chemistry
dc.subject.meshDNA, Fungal --isolation & purification
dc.subject.meshGlycoside Hydrolases --chemistry
dc.subject.meshMolecular Weight
dc.subject.meshPolysaccharides --chemistry
dc.subject.meshPseudomonas aeruginosa --enzymology
dc.subject.meshSubstrate Specificity
dc.subject.meshTemperature
dc.titlePotential application of β-1, 3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction.en_US
dc.typeArticleen_US
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