Individual donor nucleic acid testing for blood safety against HIV-1 and hepatitis B and C viruses in a tertiary care hospital.

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dc.contributor.authorCHATTERJEE, K
dc.contributor.authorCOSHIC, P
dc.contributor.authorBORGOHAIN, M
dc.contributor.authorPREMCHAND, R M
dc.contributor.authorTHAPLIYAL
dc.contributor.authorCHAKROBORTY, S
dc.contributor.authorSUNDER, S
dc.date.accessioned2015-02-23T11:23:52Z
dc.date.available2015-02-23T11:23:52Z
dc.date.issued2012-07
dc.description.abstractBackground. In July 2010, we started universal individual donor nucleic acid testing (ID-NAT) at our blood bank. This test simultaneously detects human immunodeficiency virus-1 (HIV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV) in samples of donor blood. We continued to do the enzymelinked immunosorbent assay (ELISA) test for these agents, as per the guidelines of the Drug Controller General of India. We assessed the impact of ID-NAT in preventing transfusionassociated transmission of viruses. Methods. We used fourth generation ELISA to screen blood samples of all voluntary and replacement blood donors. ID-NAT was done by transcription-mediated amplification (TMA). Results. Of the 18 356 donors, ID-NAT could not be performed on 2 samples which were inadequate. Of the 18 354 donors tested by both ID-NAT and fourth generation ELISA, 7 were found to be NAT-positive but ELISA-negative (NAT yield) for HBV and HCV. The prevalence of NAT yield cases among routine donors was 1 in 2622 donations tested (0.038%). Since we supply blood as components (packed red cells, fresh frozen plasma and platelet concentrate), these 7 units of blood would have yielded 21 components and hence 21 patients could have been infected with HBV and HCV viruses. Conclusion. In the vast majority of blood units tested, the results of ELISA and ID-NAT for HIV-1, HBV and HCV were concordant. ID-NAT did detect the presence of viruses missed by ELISA in some blood units. It widespread use in blood banks would ensure safer blood transfusion.en_US
dc.identifier.citationCHATTERJEE K, COSHIC P, BORGOHAIN M, PREMCHAND, THAPLIYAL R M, CHAKROBORTY S, SUNDER S. Individual donor nucleic acid testing for blood safety against HIV-1 and hepatitis B and C viruses in a tertiary care hospital. National Medical Journal of India. 2012 Jul-Aug ; 25(4): 207-209.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/156275
dc.language.isoenen_US
dc.source.urihttps://nmji.in/archives/Volume-25/Issue-4/Short-Report.pdfen_US
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshBlood Banks --standards
dc.subject.meshBlood Donors
dc.subject.meshBlood Specimen Collection
dc.subject.meshDNA, Viral --blood
dc.subject.meshHIV-1 --genetics
dc.subject.meshHIV-1 --isolation & purification
dc.subject.meshHepacivirus --genetics
dc.subject.meshHepacivirus --isolation & purification
dc.subject.meshHepatitis B virus --genetics
dc.subject.meshHepatitis B virus --isolation & purification
dc.subject.meshHumans
dc.subject.meshMiddle Aged
dc.subject.meshRNA, Viral --blood
dc.subject.meshTertiary Care Centers --standards
dc.subject.meshYoung Adult
dc.titleIndividual donor nucleic acid testing for blood safety against HIV-1 and hepatitis B and C viruses in a tertiary care hospital.en_US
dc.typeArticleen_US
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