Uterine Fibroid: Isolation, Culture and Characterization
Date
2019-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SB Publications
Abstract
Present study was carried out to establish uterine fibroid primary culturesystem for screening of natural/synthetic compounds against uterine fibroid. For invitro culture, enzymatic isolation method was used. To characterize, histochemistry (H& E, Masson’s Trichrome and Periodic Acid Schiff) staining and immunocytochemistryusing marker antibodies (Versican) were performed in vitro. Uterine fibroid tissueshowed much intense staining of Masson’s Trichrome and Periodic Acid Schiff stain ascompared to adjacent myometrium tissue. The primary cultured cells showedsignificantly higher proliferation, sub-culture efficiency and expression of Versicanprotein. In conclusion, our results suggest that in vitro cultured uterine fibroid cells mayoffer a suitable alternative model to evaluate natural or synthetic compounds havingantitumor properties for uterine fibroid treatment.
Description
Keywords
Uterine Fibroid, Primary Culture, mmunocytochemistry, Antitumor, Uterine leiomyoma, Versican protein
Citation
Pandey Vivek, Rani Anjali, Dubey Pawan K.. Uterine Fibroid: Isolation, Culture and Characterization. Journal of Biological Engineering Research and Review. 2019 Jul; 6(2): 27-31