Somatropin promotes dermatophagoides farinae-specific IgE generation independently of IL-4 and IL-10.

Simple item page
dc.contributor.authorNoma, Takeshien_US
dc.contributor.authorYoshizawa, Izumien_US
dc.contributor.authorSugano, Sumioen_US
dc.contributor.authorOgawa, Norifumien_US
dc.contributor.authorKawano, Yutakaen_US
dc.date.accessioned2009-05-27T17:21:05Z
dc.date.available2009-05-27T17:21:05Z
dc.date.issued2004-12-30en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractInterleukin (IL)-10 accelerates the IgE production of anti-CD40- and IL-4-stimulated PBMC by enhancing the IL-6 production of T lymphocytes or antigen-primed spleen cells, in addition to its role as a regulator of the inflammatory responses. To further investigate the mechanisms enhancing IgE synthesis, we determined the effect of somatropin as well as IL-10 on the secretion of Dermatophagoides farinae (Df)-specific IgE by K7 cells, which originate from an EBV-immortalized cell line. Df-pulsed autologous T cells, as well as the supernatants of these cultures, increased the synthesis of Df-specific IgE. Antigen-specific IgE was also enhanced when K7 cells were treated with anti-CD40 antibody and with both IL-4 and IL-10, or with IL-4 and IL-10 without anti-CD40 antibody. The treatment of K7 cells with anti-CD40 antibody and IL-4, or anti-CD40 antibody and IL-10 did not increase IgE production. The Df-specific IgE activity of the supernatants of K7 cells treated with somatropin alone was increased significantly although somatropin did not show any additive effect on the IgE production of anti-CD40 antibody-treated cells. The results indicate that IL-10, a Th2-type cytokine, directly affects the mature B cells that produce IgE, and that the secretion of IgE is increased by treatment with IL-10 in cells that are stimulated with anti-CD40 and IL-4 at the level of the EBV-immortalized cell line, which has already switched to IgE production. Somatropin similarly stimulates activated mature B cells to enhance their production of antigen-specific IgE without class switching, independently of IL-4 and IL-10.en_US
dc.description.affiliationDepartment of Pediatrics, Kitasato University School of Medicine, Kanagawa, Japan.en_US
dc.identifier.citationNoma T, Yoshizawa I, Sugano S, Ogawa N, Kawano Y. Somatropin promotes dermatophagoides farinae-specific IgE generation independently of IL-4 and IL-10. Asian Pacific Journal of Allergy and Immunology. 2004 Jun-Sep; 22(2-3): 133-42en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/37002
dc.language.isoengen_US
dc.subject.meshAntigens, CD40 --immunologyen_US
dc.subject.meshAntigens, Dermatophagoides --immunologyen_US
dc.subject.meshAsthma --immunologyen_US
dc.subject.meshB-Lymphocytes --drug effectsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshChilden_US
dc.subject.meshDermatophagoides farinae --immunologyen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGrowth Hormoneen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin E --biosynthesisen_US
dc.subject.meshInterleukin-10 --immunologyen_US
dc.subject.meshInterleukin-4 --immunologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshT-Lymphocytes --drug effectsen_US
dc.titleSomatropin promotes dermatophagoides farinae-specific IgE generation independently of IL-4 and IL-10.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Asian Pacific Journal of Allergy and Immunology