Antibacterial & antiplasmid activities of Helicteres isora L.

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dc.contributor.authorShriram, Varsha
dc.contributor.authorJahagirdar, Sheetal
dc.contributor.authorLatha, C
dc.contributor.authorKumar, Vinay
dc.contributor.authorDhakephalkar, Prashant
dc.contributor.authorRojatkar, Supada
dc.contributor.authorShitole, Mahadeo G
dc.date.accessioned2011-11-30T04:59:37Z
dc.date.available2011-11-30T04:59:37Z
dc.date.issued2010-07
dc.description.abstractBackground & objectives: The multiple drug resistance (MDR) is a serious health problem and major challenge to the global drug discovery programmes. Most of the genetic determinants that confer resistance to antibiotics are located on R-plasmids in bacteria. The present investigation was undertaken to investigate the ability of organic extract of the fruits of Helicteres isora to cure R-plasmids from certain clinical isolates. Methods: Active fractions demonstrating antibacterial and antiplasmid activities were isolated from the acetone extracts of shade dried fruits of H. isora by bioassay guided fractionation. Minimal inhibitory concentration (MIC) of antibiotics and organic extracts was determined by agar dilution method. Plasmid curing activity of organic fractions was determined by evaluating the ability of bacterial colonies (pre treated with organic fraction for 18 h) to grow in the presence of antibiotics. The physical loss of plasmid DNA in the cured derivatives was further confirmed by agarose gel electrophoresis. Results: The active fraction did not inhibit the growth of either the clinical isolates or the strains harbouring reference plasmids even at a concentration of 400 μg/ml. However, the same fraction could cure plasmids from Enterococcus faecalis, Escherichia coli, Bacillus cereus and E. coli (RP4) at curing efficiencies of 14, 26, 22 and 2 per cent respectively. The active fraction mediated plasmid curing resulted in the subsequent loss of antibiotic resistance encoded in the plasmids as revealed by antibiotic resistance profile of cured strains. The physical loss of plasmid was also confirmed by agarose gel electrophoresis. Interpretation & conclusions: The active fraction of acetone extract of H. isora fruits cured R-plasmids from Gram-positive and Gram-negative clinical isolates as well as reference strains. Such plasmid loss reversed the multiple antibiotic resistance in cured derivatives making them sensitive to low concentrations of antibiotics. Acetone fractions of H. isora may be a source to develop antiplasmid agents of natural origin to contain the development and spread of plasmid borne multiple antibiotic resistance.en_US
dc.identifier.citationShriram Varsha, Jahagirdar Sheetal, Latha C, Kumar Vinay, Dhakephalkar Prashant, Rojatkar Supada, Shitole Mahadeo G. Antibacterial & antiplasmid activities of Helicteres isora L. Indian Journal of Medical Research. 2010 Jul; 132(1): 94-99.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135546
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2010/july/0716.pdfen_US
dc.subjectAntibacterial activityen_US
dc.subjectantibiotic resistanceen_US
dc.subjectantiplasmid activityen_US
dc.subjectHelicteres isoraen_US
dc.subjectmultiple drug resistanceen_US
dc.subjectplasmid-curingen_US
dc.subject.meshAcetone
dc.subject.meshBacillus cereus --drug effects
dc.subject.meshBacillus cereus --genetics
dc.subject.meshChemical Fractionation
dc.subject.meshDrug Resistance, Multiple --genetics
dc.subject.meshElectrophoresis, Agar Gel
dc.subject.meshEnterococcus faecalis --drug effects
dc.subject.meshEnterococcus faecalis --genetics
dc.subject.meshEscherichia coli --drug effects
dc.subject.meshEscherichia coli --genetics
dc.subject.meshFruit --chemistry
dc.subject.meshIndia
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshR Factors --drug effects
dc.subject.meshR Factors --genetics
dc.subject.meshSterculiaceae --chemistry
dc.titleAntibacterial & antiplasmid activities of Helicteres isora L.en_US
dc.typeArticleen_US
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