Characterization of a Plasmodium falciparum epitope recognized by a monoclonal antibody with broad isolate and species specificity.

Simple item page
dc.contributor.authorLimpaiboon, Ten_US
dc.contributor.authorTaylor, D Wen_US
dc.contributor.authorJones, Gen_US
dc.contributor.authorGeysen, H Men_US
dc.contributor.authorSaul, Aen_US
dc.date.accessioned2009-05-27T15:15:32Z
dc.date.available2009-05-27T15:15:32Z
dc.date.issued1990-09-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractMonoclonal antibody (MAb) 7H8 raised against Plasmodium yoelii reacted with a series of proteins from P. falciparum that range in molecular weight from 46 to 194 kDa. By immunofluorescence assay, this MAb reacted with all isolates of P. falciparum tested. MAb 7H8 was used to screen a genomic expression library of asexual blood stage antigens of P. falciparum, Malayan Camp K+ and 7 independent clones were identified. These 7 clones were sequenced and the epitope recognized by MAb 7H8 in the recombinant protein of one of these clones was mapped. This epitope contained Lys Tyr Pro as core amino acids. However, similar sequences were not found in the other clones, indicating that this MAb binds to a structural epitope formed by different amino acids. The variable composition of the epitope may account for the number of P. falciparum malarial proteins recognized by MAb 7H8.en_US
dc.description.affiliationQueensland Institute of Medical Research, Herston, Brisbane, Australia.en_US
dc.identifier.citationLimpaiboon T, Taylor DW, Jones G, Geysen HM, Saul A. Characterization of a Plasmodium falciparum epitope recognized by a monoclonal antibody with broad isolate and species specificity. The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Sep; 21(3): 388-96en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32324
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal --immunologyen_US
dc.subject.meshAntigens, Protozoan --geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshClone Cellsen_US
dc.subject.meshEpitopes --geneticsen_US
dc.subject.meshMalaria --immunologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPlasmodium falciparum --geneticsen_US
dc.subject.meshRecombinant Proteins --geneticsen_US
dc.titleCharacterization of a Plasmodium falciparum epitope recognized by a monoclonal antibody with broad isolate and species specificity.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, P.H.S.en_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Southeast Asian Journal of Tropical Medicine and Public Health