Isolation and purification of penicillin G acylase obtained from Escherichia coli (NCIM-2400) and immobilisation on Eupergit C for the production of 6 amino penicillanic acid.

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dc.contributor.authorHegde, M Men_US
dc.contributor.authorThadani, S Ben_US
dc.contributor.authorSingh, Uen_US
dc.contributor.authorNaik, S Ren_US
dc.date.accessioned1997-02-01en_US
dc.date.accessioned2009-05-27T04:15:21Z
dc.date.available1997-02-01en_US
dc.date.available2009-05-27T04:15:21Z
dc.date.issued1997-02-01en_US
dc.description.abstractPenicillin G-Acylase is produced by submerged cultivation of E. Coli (NCIM-2400) and extracted from the harvested fermented broth, purified (affinity chromatography) and immobilised on Eupergit C (Synthetic polymer in bead form). The immobilised penicillin G acylase properties are studied and compared with soluble penicillin G-acylase. The control parameters for conversion of penicillin G-K to 6 APA are optimised [e.g. substrate (Pen G-K) concentration ratio to immobilised penicillin G-acylase, temperature, pH etc.] in a stirred tank reactor. Our findings suggest that immobilised penicillin G-acylase can be used commercially and the productivity of 1 kg. of immobilised enzyme is around 400 kg of 6 APA under given desired stipulated conditions.en_US
dc.description.affiliationHindustan Antibiotics Ltd., Pimpri, Pune, India.en_US
dc.identifier.citationHegde MM, Thadani SB, Singh U, Naik SR. Isolation and purification of penicillin G acylase obtained from Escherichia coli (NCIM-2400) and immobilisation on Eupergit C for the production of 6 amino penicillanic acid. Hindustan Antibiotics Bulletin. 1997 Feb-Nov; 39(1-4): 1-10en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/2619
dc.language.isoengen_US
dc.subject.meshEnzymes, Immobilized --chemistryen_US
dc.subject.meshEscherichia coli --enzymologyen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshPenicillanic Acid --analogs & derivativesen_US
dc.subject.meshPenicillin Amidase --isolation & purificationen_US
dc.subject.meshPolymersen_US
dc.titleIsolation and purification of penicillin G acylase obtained from Escherichia coli (NCIM-2400) and immobilisation on Eupergit C for the production of 6 amino penicillanic acid.en_US
dc.typeJournal Articleen_US
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