Serodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody.

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dc.contributor.authorThepthai, Charinen_US
dc.contributor.authorSmithtikarn, Saijaien_US
dc.contributor.authorSuksuwan, Montanaen_US
dc.contributor.authorSongsivilai, Sirirurgen_US
dc.contributor.authorDharakul, Tararajen_US
dc.date.accessioned2009-05-27T17:18:00Z
dc.date.available2009-05-27T17:18:00Z
dc.date.issued2005-12-29en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractBurkholderia pseudomallei is the causative agent of melioidosis, a severe and potentially fatal infectious disease in humans known to be endemic in Southeast Asia and northern Australia. The infection is also increasingly recognized in various animal species with a potential to spread to humans. With the potential as a biological warfare agent, specific serodiagnosis of melioidosis for surveillance in large populations at risk, humans or animals, would be highly valuable. In this study, a competitive enzyme-linked immunosorbent assay (ELISA) using a lipopolysaccharide-specific monoclonal antibody was developed. The assay provides high specificity, based on a previously described monoclonal antibody to a specific epitope on the lipopolysaccharide (LPS) of B. pseudomallei. The assay sensitivity of 96.0% and specificity of 100% were achieved at a cutoff value of 50% inhibition in human culture-proven melioidosis cases. An optimal cutoff value of 65% inhibition for sera from a melioidosis endemic area was obtained by ROC analysis and resulted in an assay specificity of 86.2%, while maintaining assay sensitivity of 92.0%. A potential application of the assay in the serodiagnosis of melioidosis in animal species was also evaluated usina dolphin sera with satisfactory results.en_US
dc.description.affiliationDepartment of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationThepthai C, Smithtikarn S, Suksuwan M, Songsivilai S, Dharakul T. Serodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody. Asian Pacific Journal of Allergy and Immunology. 2005 Jun-Sep; 23(2-3): 127-32en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36888
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Bacterial --immunologyen_US
dc.subject.meshAntibodies, Monoclonal --diagnostic useen_US
dc.subject.meshAntigen-Antibody Reactions --immunologyen_US
dc.subject.meshAntigens, Bacterial --immunologyen_US
dc.subject.meshBurkholderia pseudomallei --immunologyen_US
dc.subject.meshEndemic Diseasesen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshLipopolysaccharides --immunologyen_US
dc.subject.meshMelioidosis --diagnosisen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSerologic Testsen_US
dc.subject.meshThailand --epidemiologyen_US
dc.titleSerodiagnosis of melioidosis by a competitive enzyme-linked immunosorbent assay using a lipopolysaccharide-specific monoclonal antibody.en_US
dc.typeComparative Studyen_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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