Alkaline lipase production by Citrobacter freundii IIT-BT L139.

Simple item page
dc.contributor.authorGunasekaran, Vijayen_US
dc.contributor.authorKotay, Shireen Meheren_US
dc.contributor.authorDas, Debabrataen_US
dc.date.accessioned2009-05-28T15:12:09Z
dc.date.available2009-05-28T15:12:09Z
dc.date.issued2006-06-21en_US
dc.description.abstractAround 150 lipase producing bacterial isolates were screened from the local soils enriched with oil. Citrobacrer freundii IIT-BT L139, an isolated microbial strain, produced lipase that had high activity (8.8 U/ml) at pH 9.0 and 40 degrees C. The 16S rDNA phylogenetic studies showed that Citrobacter freundii belongs to the family Enterobacteriaceae and later confirmed by the microbial identification. Suitable C and N sources for lipase production were deduced to be starch and peptone-urea, respectively. In a controlled fermenter (1 L), the lipase activity was found to increase by 36% (12 Uml(-1)). The variation of lipase activity, pH and dissolved oxygen (DO) during growth of the organism in the controlled batch fermenter were monitored. The rheological characteristics of the fermentation broth indicated that it behaved like a Newtonian fluid throughout the fermentation. The fermentation time was comparatively short (60 h). The lipase was also found to be substantially resistant to common detergents. This lipase was, thus, characterized as alkaline, thermostable and solvent stable, which was essentially desirable in pharmaceutical, detergent and other industrial applications or production.en_US
dc.description.affiliationDepartment of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.en_US
dc.identifier.citationGunasekaran V, Kotay SM, Das D. Alkaline lipase production by Citrobacter freundii IIT-BT L139. Indian Journal of Experimental Biology. 2006 Jun; 44(6): 485-91en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/61685
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/6516/1/IJEB%2044%286%29%20485-491.pdfen_US
dc.subject.meshCitrobacter freundii --enzymologyen_US
dc.subject.meshDNA, Ribosomal --chemistryen_US
dc.subject.meshEnzyme Stabilityen_US
dc.subject.meshFermentationen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshLipase --biosynthesisen_US
dc.subject.meshLipid Metabolismen_US
dc.subject.meshOxygen --metabolismen_US
dc.subject.meshPhylogenyen_US
dc.subject.meshRheologyen_US
dc.subject.meshSolvents --chemistryen_US
dc.subject.meshSurface-Active Agents --chemistryen_US
dc.subject.meshTemperatureen_US
dc.subject.meshTime Factorsen_US
dc.titleAlkaline lipase production by Citrobacter freundii IIT-BT L139.en_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Experimental Biology