Quinacrine causes apoptosis in human cancer cell lines through caspase-mediated pathway and regulation of small-GTPase

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dc.contributor.authorSamanta, Angelaen_US
dc.contributor.authorRavindran, Geethanjalen_US
dc.contributor.authorSarkar, Angshumanen_US
dc.date.accessioned2020-11-18T10:14:22Z
dc.date.available2020-11-18T10:14:22Z
dc.date.issued2020-02
dc.description.abstractQuinacrine (QC), an FDA-approved anti-malarial drug, has shown to have anticancer activities. Due to its‘shotgun’ nature, QC has become an inevitable candidate for combination chemotherapy. There is lack of studyof the molecular interplay between colorectal cancer (CRC) microenvironment and its metastasis. In this study,we focused on the differential anti-cancerous effect of QC on two different human cancer cell lines, HCT 116and INT 407. Results suggest that cytotoxicity increased in both the cell lines with an increase in QCconcentration. The expression patterns of small-GTPases and caspases were altered significantly in QC-treatedcells compared to non-treated cells. HSP70 and p53 showed comparable differences in the expression pattern.The wound-healing assay showed an increase in the denuded zone, with an increase in the concentration ofQC. The formation of apoptotic nuclei increased with a rise in the concentration of QC in both the cell lines.The decrease and increase in caspase 9 and caspase 3 expression respectively were studied, confirmingapoptosis by the extrinsic pathwayen_US
dc.identifier.affiliationsCMBL, Department of Biological Sciences, BITS Pilani K K Birla Goa Campus, Zuarinagar 403 726, Indiaen_US
dc.identifier.affiliationsCentre for Cellular and Molecular Biology, Habsiguda, Uppal Road, Hyderabad 500 007, Indiaen_US
dc.identifier.affiliationsCMBL, Department of Biological Sciences, BITS Pilani K K Birla Goa Campus, Zuarinagar 403 726, Indiaen_US
dc.identifier.citationSamanta Angela, Ravindran Geethanjal, Sarkar Angshuman. Quinacrine causes apoptosis in human cancer cell lines through caspase-mediated pathway and regulation of small-GTPase. Journal of Biosciences. 2020 Feb; : 1-18en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214321
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume45en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-020-0011-3en_US
dc.subjectCaspase 3en_US
dc.subjectcaspase 8en_US
dc.subjectcaspase 9en_US
dc.subjectHCT 116en_US
dc.subjectHsp70en_US
dc.subjectINT 407en_US
dc.subjectp53en_US
dc.subjectQuinacrineen_US
dc.subjectsmall GTPasesen_US
dc.titleQuinacrine causes apoptosis in human cancer cell lines through caspase-mediated pathway and regulation of small-GTPaseen_US
dc.typeJournal Articleen_US
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