Angiostrongyliasis: analysis of antigens of Angiostrongylus costaricensis adult worms versus IgG from infected patients with Angiostrongylus cantonensis.

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dc.contributor.authorDekumyoy, Pen_US
dc.contributor.authorKomalamisra, Cen_US
dc.contributor.authorNuamtanong, Sen_US
dc.contributor.authorNacapunchai, Den_US
dc.contributor.authorSinnawong, Men_US
dc.contributor.authorShanaha, Pen_US
dc.contributor.authorPiyasatittam, Pen_US
dc.date.accessioned2009-05-27T15:10:52Z
dc.date.available2009-05-27T15:10:52Z
dc.date.issued2000-03-21en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe possibility of cross-reactivity was previously investigated by indirect ELISA with sera from Angiostrongylus cantonensis infections, normal controls and A. costaricensis antigen. 5 microg/ml of crude antigen from both sexes of each species reacted with diluted serum samples (1:800) of each of 20 cases of angiostrongyliasis and normal controls, and further with anti-human IgG conjugate at 1:1,000. The mean absorbance values were evaluated as follows; normal controls showed a value of 0.033 using A. costaricensis antigen lower than (0.085) A. costaricensis antigen. Both mean values of angiostrongyliasis cases were rather close (0.491) using A. costaricensis antigen and the other antigen (0.518). The present study continued with a crude antigen of 13 A. costaricensis females and males. Serum samples were analyzed; 27 sera of angiostrongyliasis, 30 negative controls and 193 cases of other parasitic infections (91 cases of nematodiasis; 45 cases of cestodiasis; 47 cases of trematodiasis and 10 cases of HIV) and 7 cases of other brain infections. This antigen was evaluated for ELISA with a concentration of 5 microg/ml, serum dilution 1:400 and anti-human IgG conjugate at 1:2,000. The test gave sensitivity and specificity at cut-off value 0.261; 92.59% and 73% respectively. The antigen was cross-reactive with 30 cases from 9 out of 10 different kinds of nematodiasis (gnathostomiasis, strongyloidiasis, ascariasis, hookworm infections, trichinosis, toxocariasis, trichuriasis, onchocercosis and Wuchereria bancrofti infections. Five cases from 3 of 6 kinds of cestodiasis (neurocysticercosis, echinococcosis and Hymenolepis nana infections) and 18 cases of 4 out of 5 kinds of trematodiasis (Paragonimus heterotremus infections, opisthorchiasis, schistosomiasis and fascioliasis). One case of other brain infections was observed. The crude antigen of A. costaricensis showed a high percentage sensitivity with serum antibodies of angiostrongyliasis cases. Low specificity of the test was observed by reactions of those serum antibodies with various kinds of antigenic molecules. This study provides baseline data for further immunodiagnosis of human angiostrongyliasis.en_US
dc.description.affiliationDepartment of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationDekumyoy P, Komalamisra C, Nuamtanong S, Nacapunchai D, Sinnawong M, Shanaha P, Piyasatittam P. Angiostrongyliasis: analysis of antigens of Angiostrongylus costaricensis adult worms versus IgG from infected patients with Angiostrongylus cantonensis. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 ; 31 Suppl 1(): 48-53en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32124
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAngiostrongylus cantonensis --immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Helminth --isolation & purificationen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshStrongylida Infections --immunologyen_US
dc.titleAngiostrongyliasis: analysis of antigens of Angiostrongylus costaricensis adult worms versus IgG from infected patients with Angiostrongylus cantonensis.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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