Diagnosis of Cryptococcosis in Dogs by Latex Agglutination Test and Enzyme Immunoassay

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dc.contributor.authorSharma, Sahilen_US
dc.contributor.authorGupta, Paviteren_US
dc.contributor.authorSharma, N.S.en_US
dc.contributor.authorArora, A.K.en_US
dc.contributor.authorChaabra, Sushmaen_US
dc.contributor.authorRai, T.S.en_US
dc.date.accessioned2020-10-16T08:58:47Z
dc.date.available2020-10-16T08:58:47Z
dc.date.issued2019-08
dc.description.abstractCryptococcus spp. is a pathogenic fungus which is an increasingly important cause of infection, particularly in the immunocompromised hosts. Diagnosis of cryptococcosis in animals can be carried out by isolation of the fungus but this requires several days to detect and identify the organism. Detection of cryptococcal antigen by latex agglutination test and enzyme immunoassay in serum is a rapid and easy method for diagnosis of cryptococcosis. In the present study, a total of 142 blood samples were collected from apparently healthy (n=89) and diseased dogs (n=53) for diagnosis of cryptococcosis. Latex agglutination test and enzyme immunosorbent assay (EIA) were carried out for the detection of cryptococcal antigen in serum. Of the 142 serum samples tested, six samples tested positive by Latex agglutination test while one sample tested positive by EIA. The sample which was positive by EIA was also positive by Latex agglutination test. The serum samples of dogs that tested positive for cryptococcal antigen were obtained from dogs suffering from symptoms like bloody faeces and vomit, emesis, chronic ear infection and discharge. Based on our findings, we conclude that the latex agglutination test in combination with the enzyme immunoassay can be used for the diagnosis of Cryptococcosis in dogsen_US
dc.identifier.affiliationsDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIAen_US
dc.identifier.affiliationsDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIAen_US
dc.identifier.affiliationsDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIAen_US
dc.identifier.affiliationsDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIAen_US
dc.identifier.affiliationsDepartment of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIAen_US
dc.identifier.affiliationsDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, INDIAen_US
dc.identifier.citationSharma Sahil, Gupta Paviter, Sharma N.S., Arora A.K., Chaabra Sushma, Rai T.S.. Diagnosis of Cryptococcosis in Dogs by Latex Agglutination Test and Enzyme Immunoassay. Journal of Animal Research. 2019 Aug; 9(4): 505-510en_US
dc.identifier.issn2249-6629
dc.identifier.issn2277-940X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/210844
dc.languageenen_US
dc.publisherAssociation of Mastitisen_US
dc.relation.issuenumber4en_US
dc.relation.volume9en_US
dc.source.urihttps://dx.doi.org//10.30954/2277-940X.04.2019.2en_US
dc.subjectCryptococcusen_US
dc.subjectblooden_US
dc.subjectdogsen_US
dc.subjectlatex agglutination testen_US
dc.subjectenzyme immunoassayen_US
dc.titleDiagnosis of Cryptococcosis in Dogs by Latex Agglutination Test and Enzyme Immunoassayen_US
dc.typeJournal Articleen_US
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