A Novel Validated Uhplc Method For Estimation Of Assay And Its Related Substances Of Trichostatin-A

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dc.contributor.authorRao, L. V.en_US
dc.contributor.authorRao, K. T.en_US
dc.contributor.authorKandepi, V. V. K. M.en_US
dc.date.accessioned2020-09-24T08:01:50Z
dc.date.available2020-09-24T08:01:50Z
dc.date.issued2020-07
dc.description.abstractObjective: The main objective of the research work is to develop and validate a rapid UHPLC method for the estimation of assay and its related substances of Trichostatin A (TSA) in pharmaceutical samples. Methods: The UHPLC method developed for chromatographic separation between TSA and its related compounds on Poroshell 120 SB C18(50×4.6) mm; 2.7 µm RRLC column using Agilent RRLC (UHPLC) system with linear gradient elution. Results: The developed UHPLC method has shown excellent chromatographic separation between TSA and its related compounds within 12 min run time, during validation experiments, specificity study revealed that the peak threshold was more than the peak purity and no purity flag was observed. Repeatability, intra, and inter-day precision results were well within the tolerable limits. Limits of detection concentrations were found between 0.075 to 0.077 ppm and the limit of quantitation is between 0.252 to 0.258 ppm for related compounds and TSA. The related substances method recoveries were found between 80 and 120 % and assay method recovery was found between 98.0 to 102.0%. Conclusion: The developed method capability was proven for the assay of TSA and its related compounds in pharmaceutical samples and the method shows eco-friendlier than routine, conventional HPLC methods in terms of analysis time, cost and HPLC effluent waste.en_US
dc.identifier.affiliationsDepartment of Chemistry, Gitam Institute of Science, GITAM University (Deemed to be), Visakhapatnam 530045, Indiaen_US
dc.identifier.affiliationsDepartment of Chemistry, Gitam Institute of Science, GITAM University (Deemed to be), Visakhapatnam 530045, India, Product and Technology Development, Eco Logic Technologies Ltd, Hyderabad 500072, Indiaen_US
dc.identifier.affiliationsProduct and Technology Development, Eco Logic Technologies Ltd, Hyderabad 500072, Indiaen_US
dc.identifier.citationRao L. V., Rao K. T., Kandepi V. V. K. M. . A Novel Validated Uhplc Method For Estimation Of Assay And Its Related Substances Of Trichostatin-A. International Journal of Pharmacy and Pharmaceutical Sciences. 2020 Jul; 12(7): 45-50en_US
dc.identifier.issn0975-1491
dc.identifier.issn2656-0097
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/206124
dc.languageenen_US
dc.publisherInnovare Academic Sciences Pvt. Ltd.en_US
dc.relation.issuenumber7en_US
dc.relation.volume12en_US
dc.source.urihttps://doi.org/10.22159/ijpps.2020v12i7.37198en_US
dc.subjectTrichostatin Aen_US
dc.subjectAssayen_US
dc.subjectRelated substancesen_US
dc.subjectMethod Validationen_US
dc.subjectRRLCen_US
dc.subjectUHPLCen_US
dc.titleA Novel Validated Uhplc Method For Estimation Of Assay And Its Related Substances Of Trichostatin-Aen_US
dc.typeJournal Articleen_US
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