Programmed cell death in the larval salivary glands of Apis mellifera (Hymenoptera, Apidae).

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dc.contributor.authorSilva-Zacarin, E C Men_US
dc.contributor.authorTomaino, G Aen_US
dc.contributor.authorBrocheto-Braga, M Ren_US
dc.contributor.authorTaboga, S Ren_US
dc.contributor.authorDe Moraes, R L M Silvaen_US
dc.date.accessioned2007-03-17en_US
dc.date.accessioned2009-06-01T14:21:52Z
dc.date.available2007-03-17en_US
dc.date.available2009-06-01T14:21:52Z
dc.date.issued2007-03-17en_US
dc.description.abstractThe morphological and histochemical features of degeneration in honeybee (Apis mellifera) salivary glands were investigated in 5th instar larvae and in the pre-pupal period. The distribution and activity patterns of acid phosphatase enzyme were also analysed. As a routine,the larval salivary glands were fixed and processed for light microscopy and transmission electron microscopy.Tissue sections were subsequently stained with haematoxylin -eosin,bromophenol blue,silver,or a variant of the critical electrolyte concentration (CEC) method.Ultrathin sections were contrasted with uranyl acetate and lead citrate.Glands were processed for the histochemical and cytochemical localization of acid phosphatase,as well as biochemical assay to detect its activity pattern. Acid phosphatase activity was histochemically detected in all the salivary glands analysed.The cytochemical results showed acid phosphatase in vesicles, Golgi apparatus and lysosomes during the secretory phase and,additionally, in autophagic structures and luminal secretion during the degenerative phase. These findings were in agreement with the biochemical assay. At the end of the 5th instar, the glandular cells had a vacuolated cytoplasm and pyknotic nuclei, and epithelial cells were shed into the glandular lumen.The transition phase from the 5th instar to the pre-pupal period was characterized by intense vacuolation of the basal cytoplasm and release of parts of the cytoplasm into the lumen by apical blebbing; these blebs contained cytoplasmic RNA, rough endoplasmic reticule and, occasionally, nuclear material. In the pre-pupal phase, the glandular epithelium showed progressive degeneration so that at the end of this phase only nuclei and remnants of the cytoplasm were observed.The nuclei were pyknotic,with peripheral chromatin and blebs. The gland remained in the haemolymph and was recycled during metamorphosis. The programmed cell death in this gland represented a morphological form intermediate between apoptosis and autophagy.en_US
dc.description.affiliationUniversidade Federal de Sao Carlos-UFSCar, Avenida Darci Carvalho Daffener, 200 Alto da Boa Vista, Sorocaba/SP, Brasil. elaine@power.ufscar.bren_US
dc.identifier.citationSilva-Zacarin EC, Tomaino GA, Brocheto-Braga MR, Taboga SR, De Moraes RL. Programmed cell death in the larval salivary glands of Apis mellifera (Hymenoptera, Apidae). Journal of Biosciences. 2007 Mar; 32(2): 309-28en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110776
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAcid Phosphatase --metabolismen_US
dc.subject.meshAnalysis of Varianceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosis --physiologyen_US
dc.subject.meshBees --physiologyen_US
dc.subject.meshHistocytochemistryen_US
dc.subject.meshLarva --physiologyen_US
dc.subject.meshMetamorphosis, Biological --physiologyen_US
dc.subject.meshMicroscopy, Electron, Transmissionen_US
dc.subject.meshSalivary Glands --ultrastructureen_US
dc.titleProgrammed cell death in the larval salivary glands of Apis mellifera (Hymenoptera, Apidae).en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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