Effect of simultaneous exposure to lead and cadmium on gonadotropin binding and steroidogenesis on granulosa cells: an in vitro study.

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dc.contributor.authorPriya, P N Laxmien_US
dc.contributor.authorPillai, Anilen_US
dc.contributor.authorGupta, Saritaen_US
dc.date.accessioned2009-05-28T14:23:17Z
dc.date.available2009-05-28T14:23:17Z
dc.date.issued2004-02-31en_US
dc.description.abstractEffects of lead (Pb) and cadmium (Cd) both alone or in combination on the binding of LH and FSH on isolated granulosa cells were studied. Granulosa cells isolated from proestrous rats were incubated (in vitro) with lead acetate and/or cadmium acetate (0.03 microM of Pb or Cd) for 1 hr. LH binding was dropped to 84% in Pb treated cells, 72.5% in Cd treated cells and 74.8% in combined metal treated cells compared to control. FSH binding dropped to 85.5% in Pb treated cells, 71.16% in Cd treated cells and 72.5% in combined metal treated cells compared to control. Activity of 17beta Hydroxy Steroid Dehydrogenase (17betaHSDH), a key steroidogenic enzyme was reduced by 52% in Cd and 37% in combined metal exposed cells whereas Pb exposed cells showed 31% reduction in the enzyme activity. Pretreatment with SH groups protectants (glutathione [GSH], dithiothretol [DTT]) and zinc caused an ameriolation in enzyme activity whereas Zn pretreatment showed an increase in gonadotropin binding in metal exposed cells. These results suggest that both Pb and Cd can cause a reduction in LH and FSH binding, which significantly alters steroid production in vitro and exerts a direct influence on granulosa cell function.en_US
dc.description.affiliationDepartment of Biochemistry, Faculty of Science, M.S.University of Baroda, Vadodara 390 002, India.en_US
dc.identifier.citationPriya PN, Pillai A, Gupta S. Effect of simultaneous exposure to lead and cadmium on gonadotropin binding and steroidogenesis on granulosa cells: an in vitro study. Indian Journal of Experimental Biology. 2004 Feb; 42(2): 143-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60643
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCadmium --toxicityen_US
dc.subject.meshDithiothreitol --pharmacologyen_US
dc.subject.meshDrug Combinationsen_US
dc.subject.meshFemaleen_US
dc.subject.meshFollicle Stimulating Hormone --metabolismen_US
dc.subject.meshGlutathione --pharmacologyen_US
dc.subject.meshGranulosa Cells --drug effectsen_US
dc.subject.meshHydroxysteroid Dehydrogenases --metabolismen_US
dc.subject.meshLead --toxicityen_US
dc.subject.meshLuteinizing Hormone --metabolismen_US
dc.subject.meshProestrus --drug effectsen_US
dc.subject.meshRatsen_US
dc.subject.meshSteroids --biosynthesisen_US
dc.subject.meshZinc --pharmacologyen_US
dc.titleEffect of simultaneous exposure to lead and cadmium on gonadotropin binding and steroidogenesis on granulosa cells: an in vitro study.en_US
dc.typeComparative Studyen_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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