Characterization of a novel antifungal protein from Acacia senegal seeds

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dc.contributor.authorSolanki, D.S.en_US
dc.contributor.authorKumar, S.en_US
dc.contributor.authorParihar, K.en_US
dc.contributor.authorTak, A.en_US
dc.contributor.authorGehlot, P.en_US
dc.contributor.authorPathak, R.en_US
dc.contributor.authorSingh, S.K.en_US
dc.date.accessioned2020-11-18T10:16:11Z
dc.date.available2020-11-18T10:16:11Z
dc.date.issued2020-05
dc.description.abstractAim: The aim of the present study was to isolate antifungal protein from seeds of Acacia senegal in order to develop a new, effective and environmental friendly biofungicide.Methodology: Antifungal protein from A. senegal seeds was extracted and purified through ammonium sulphate precipitation, dialysis, ion exchange and gel filtration chromatography. The novel antifungal was characterized employing SDS-PAGE, chitinase activity and antifungal efficacy. The purified protein was also characterized through MALDI-TOF MS/MS. Results: The yield of purified antifungal protein was estimated to be 0.96 mg 25 g-1 seeds and its molecular mass determined by SDS PAGE was 52.9 kDa. The purified protein exhibited antifungal activity against phytopathogenic fungi viz., Macrophomina phaseolina and Fusarium oxysporum also possessed chitinase activity. The purified protein was characterized through MALDI-TOF MS/MS and its spectra revealed 14 peptides with their specific amino acid sequences. Interpretation: The antifungal protein isolated from A. senegal seeds has broad-spectrum antifungal activity with chitinase activity against pathogenic fungi that can be exploited for management of fungal disease as biopesticide to promote sustainable agricultureen_US
dc.identifier.affiliationsDepartment of Botany, Jai Narain Vyas University, Jodhpur-342 001, Indiaen_US
dc.identifier.affiliationsDepartment of Botany, Jai Narain Vyas University, Jodhpur-342 001, Indiaen_US
dc.identifier.affiliationsDepartment of Botany, Jai Narain Vyas University, Jodhpur-342 001, Indiaen_US
dc.identifier.affiliationsDepartment of Botany, Jai Narain Vyas University, Jodhpur-342 001, Indiaen_US
dc.identifier.affiliationsDepartment of Botany, Jai Narain Vyas University, Jodhpur-342 001, Indiaen_US
dc.identifier.affiliationsDivision of Plant Improvement and Pest Management, ICAR-Central Arid Zone Research Institute, Jodhpur-342 003, Indiaen_US
dc.identifier.affiliationsDivision of Plant Improvement and Pest Management, ICAR-Central Arid Zone Research Institute, Jodhpur-342 003, Indiaen_US
dc.identifier.citationSolanki D.S., Kumar S., Parihar K., Tak A., Gehlot P., Pathak R., Singh S.K.. Characterization of a novel antifungal protein from Acacia senegal seeds. Journal of Environmental Biology . 2020 May; 41(3): 607-612en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214518
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber3en_US
dc.relation.volume41en_US
dc.source.urihttps://www.jeb.co.in/journal_issues/202005_may20/paper_12.pdfen_US
dc.subjectAcacia senegalen_US
dc.subjectAntifungal proteinen_US
dc.subjectChitinaseactivityen_US
dc.subjectMALDI TOF-Mass spectroscopyen_US
dc.subjectPhytopathogenicfungien_US
dc.titleCharacterization of a novel antifungal protein from Acacia senegal seedsen_US
dc.typeJournal Articleen_US
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