Quantitation of ethyl glucuronide in serum & urine by gas chromatography - mass spectrometry.

Simple item page
dc.contributor.authorSharma, Priyamvada
dc.contributor.authorBharat, Venkatesh
dc.contributor.authorMurthy, Pratima
dc.date.accessioned2015-06-26T04:38:38Z
dc.date.available2015-06-26T04:38:38Z
dc.date.issued2015-01
dc.description.abstractBackground & objectives: Alcohol misuse has now become a serious public health problem and early intervention is important in minimizing the harm. Biochemical markers of recent and high levels of alcohol consumption can play an important role in providing feedback regarding the health consequences of alcohol misuse. Existing markers are not sensitive to recent consumption and in detecting early relapse. Ethyl glucuronide (EtG), a phase-II metabolite of ethanol is a promising marker of recent alcohol use and can be detected in body fluids. In this study an analytical technique for quantitation of EtG in body fluids using solid-phase extraction (SPE) and gas chromatography (GC) with mass spectrometric detection (MS) was developed and validated. Methods: De-proteinization of serum and urine samples was done with perchloric acid and hydrochloric acid, respectively. Serum samples were passed through phospholipids removal cartridges for further clean up. EtG was isolated using amino propyl solid phase extraction columns. Chromatographic separation was achieved by gas chromatography with mass spectrometry. Results: Limit of detection and limit of quantitation were 50 and 150 ng/ml for urine and 80 and 210 ng/ml for serum, respectively. Signal to noise ratio was 3:1, mean absolute recovery was 80-85 per cent. Significant correlation was obtained between breath alcohol and serum EtG levels (r=0.853) and urine EtG and time since last abuse (r = -0.903) in clinical samples. Interpretation & conclusions: In the absence of other standardized techniques to quantitate EtG in biological samples, this gc -ms method was found to have high throughput and was sensitive and specific.en_US
dc.identifier.citationSharma Priyamvada, Bharat Venkatesh, Murthy Pratima. Quantitation of ethyl glucuronide in serum & urine by gas chromatography - mass spectrometry. Indian Journal of Medical Research. 2015 Jan; 141 (1): 75-80.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158407
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405944/?report=classicen_US
dc.subjectEthanolen_US
dc.subjectethyl glucuronideen_US
dc.subjectgas chromatographen_US
dc.subjectmass spectrometeren_US
dc.subjectsolid phase extractionen_US
dc.subject.meshAlcohol-Induced Disorders
dc.subject.meshGas Chromatography-Mass Spectrometry
dc.subject.meshGlucuronates --analysis
dc.subject.meshGlucuronates --blood
dc.subject.meshGlucuronates --urine
dc.subject.meshHumans
dc.subject.meshSolid Phase Extraction
dc.titleQuantitation of ethyl glucuronide in serum & urine by gas chromatography - mass spectrometry.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijmr2015v141n1p75.pdf
Size:
620.27 KB
Format:
Adobe Portable Document Format
Description:
Journal artical
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Medical Research