Molecular analysis of human leukocyte antigen class I and class II allele frequencies and haplotype distribution in Pakistani population.

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dc.contributor.advisorHistocompatibility Antigens Class I --analysis
dc.contributor.authorMoatter, T
dc.contributor.authorAban, M
dc.contributor.authorTabassum, S
dc.contributor.authorShaikh, U
dc.contributor.authorPervez, S
dc.date.accessioned2012-06-11T04:32:23Z
dc.date.available2012-06-11T04:32:23Z
dc.date.issued2010-09
dc.description.abstractAIM: Distribution of HLA class I and II alleles and haplotype was studied in Pakistani population and compared with the data reported for Caucasoid, Africans, Orientals and Arab populations. MATERIALS AND METHODS: HLA class I and II polymorphisms in 1000 unrelated Pakistani individuals was studied using sequence-specific primers and polymerase chain reaction and assay. RESULTS: The most frequent class I alleles observed were A*02, B*35 and CW*07, with frequencies of 19.2, 13.7 and 20%, respectively. Fifteen distinct HLA-DRB1 alleles and eight HLA-DQB1 alleles were recognized. The most frequently observed DRB1 alleles which represented more than 60% of the subjects were DRB1 *03, *07, *11 and *15. The rare DRB1 alleles detected in this study were HLADRB1 *08 and *09, having frequencies of 0.9 and 1.7%, respectively. In addition, at DRB1-DQB1 loci there were 179 different haplotypes and 285 unique genotypes and the most common haplotype was DRB1*15-DQB1*06 which represented 17% of the total DRB1-DQB1 haplotypes. In our population, haplotype A*33-B*58-Cw*03 comprised 2.8% of the total class I haplotypes observed. This haplotype was seen only in the oriental populations and has not been reported in the African or European Caucasoid. CONCLUSION: Our study showed a close similarity of HLA class I and II alleles with that of European Caucasoid and Orientals. In Pakistani population, two rare loci and three haplotypes were identified, whereas haplotypes characteristic of Caucasians, Africans and Orientals were also found, suggesting an admixture of different races due to migration to and from this region.en_US
dc.identifier.citationMoatter T, Aban M, Tabassum S, Shaikh U, Pervez S. Molecular analysis of human leukocyte antigen class I and class II allele frequencies and haplotype distribution in Pakistani population. Indian Journal of Human Genetics. 2010 Sept; 16(3): 149-153.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/138915
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3009426/en_US
dc.subjectDQB1en_US
dc.subjectDRB1en_US
dc.subjecthuman leukocyte antigen polymorphismen_US
dc.subjecthuman leukocyte antigen, HLA-Aen_US
dc.subjectHLA-Ben_US
dc.subjectHLA-Cen_US
dc.subjectPakistanen_US
dc.subjectpopulation geneticsen_US
dc.subjectsequence-specific oligonucleotide analysisen_US
dc.subject.meshHistocompatibility Antigens Class I --genetics
dc.subject.meshHistocompatibility Antigens Class II --analysis
dc.subject.meshHistocompatibility Antigens Class II --genetics
dc.subject.meshHLA-B Antigens --analysis
dc.subject.meshHLA-B Antigens --genetics
dc.subject.meshHLA-C Antigens --analysis
dc.subject.meshHLA-C Antigens --genetics
dc.subject.meshHLA-DQ beta-Chains --analysis
dc.subject.meshHLA-DQ beta-Chains --genetics
dc.subject.meshHLA-DRB1 Chains --analysis
dc.subject.meshHLA-DRB1 Chains --genetics
dc.subject.meshHumans
dc.subject.meshMolecular Diagnostic Techniques
dc.subject.meshOligonucleotide Array Sequence Analysis --methods
dc.subject.meshPakistan
dc.subject.meshPolymorphism, Genetic --genetics
dc.subject.meshPopulation Groups --genetics
dc.titleMolecular analysis of human leukocyte antigen class I and class II allele frequencies and haplotype distribution in Pakistani population.en_US
dc.typeArticleen_US
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