Association of cucumovirus and potyvirus with betelvine (Piper betle L.) as evidenced by ELISA and RT-PCR.

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dc.contributor.authorRaj, S Ken_US
dc.contributor.authorSrivastava, Aen_US
dc.contributor.authorChowdhury, M Ren_US
dc.contributor.authorJohri, J Ken_US
dc.date.accessioned2009-05-28T13:55:01Z
dc.date.available2009-05-28T13:55:01Z
dc.date.issued2003-03-23en_US
dc.description.abstractAn attempt was made to detect various viruses of Piper betle grown at Mahoba and Banthara in India. DAC-ELISA and RT-PCR tests were performed in leaf sap samples of betelvine for detection of a cucumovirus (Cucumber mosaic virus) and potyvirus (Bean yellow mosaic virus) using specific antibodies and universal primers of respective viruses. DAC-ELISA could detect only CMV. However, RT-PCR detected both cucumovirus and potyvirus infection in betelvine samples. Association of CMV with betelvine was observed for the first time in the present study.en_US
dc.description.affiliationMolecular Virology, National Botanical Research Institute, Lucknow 226 001, India.en_US
dc.identifier.citationRaj SK, Srivastava A, Chowdhury MR, Johri JK. Association of cucumovirus and potyvirus with betelvine (Piper betle L.) as evidenced by ELISA and RT-PCR. Indian Journal of Experimental Biology. 2003 Mar; 41(3): 273-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60032
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAntibodies, Viral --analysisen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCucumovirus --geneticsen_US
dc.subject.meshDNA Primersen_US
dc.subject.meshDNA, Viral --analysisen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshPiper betle --virologyen_US
dc.subject.meshPotyvirus --geneticsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.titleAssociation of cucumovirus and potyvirus with betelvine (Piper betle L.) as evidenced by ELISA and RT-PCR.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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