Possible modulating action of plant infusion of Ocimum sanctum L. on chromosomal aberrations and sister chromatid exchanges induced by chlormadinone acetate in human lymphocytes in vitro.

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dc.contributor.authorSiddique, Yasir Hasanen_US
dc.contributor.authorAra, Gulshanen_US
dc.contributor.authorBeg, Tanveeren_US
dc.contributor.authorAfzal, Mohammaden_US
dc.date.accessioned2008-11-21en_US
dc.date.accessioned2009-06-02T03:46:27Z
dc.date.available2008-11-21en_US
dc.date.available2009-06-02T03:46:27Z
dc.date.issued2008-11-21en_US
dc.description.abstractChlormadinone acetate (CMA) is a synthetic progesterone analogue. It has its usage in oral contraceptives formulations and also for estrous synchronization of animals. The aim of the present study is to study the anti- genotoxic activity of the plant infusion against the CMA induced genotoxic damage on cultured human lymphocytes, using chromosomal aberrations and sister chromatid exchanges (SCFs) as parameters. For chromosomal aberration analysis, the treatment of 40 microM of CMA was associated with 4.33% abnormal metaphases. The treatment of 40 microM of CMA, separately with 1.075 x 10(-4), 2.125 x 10(-4) and 3.15 x 10(-4) gm l(-1) of plant infusion results in the reduction of the number of abnormal metaphases i.e. 2.67%, 2.00% and 1.67% respectively. For sister chromatid exchange analysis, the frequency of sister chromatid exchange per cell (SCE(S)/Cell) for the treatment of 40 microM of CMA was 6.43. The treatment of 40 microM of CMA, separately with 1.075 x 10(-4), 2.125 x 10(-4) and 3.15 x 10(-4) gm l(-1) of plant infusion results in the significant reduction of the frequency of SCE(S)/Cell i.e. 3.76, 3.01 and 2.94, respectively, as compared to the CMA (40 microM) treatment alone (6.43). The used dosages of plant infusion did not increase chromosomal aberrations and sister chromatid exchanges at significant level as compared to the untreated. The results of the present study suggest that the plant infusion per se does not have genotoxic potential, but can modulate the genotoxicity of chlormadinone acetate in human lymphocytes in vitro.en_US
dc.description.affiliationSection of Genetics, Department of Zoology, Aligarh Muslim University, Aligarh 202 002, India. yasir_hasansiddique@rediffmail.comen_US
dc.identifier.citationSiddique YH, Ara G, Beg T, Afzal M. Possible modulating action of plant infusion of Ocimum sanctum L. on chromosomal aberrations and sister chromatid exchanges induced by chlormadinone acetate in human lymphocytes in vitro. Journal of Environmental Biology. 2008 Nov; 29(6): 845-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/113302
dc.language.isoengen_US
dc.source.urihttps://www.geocities.com/j_environ_biol/en_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChlormadinone Acetate --pharmacologyen_US
dc.subject.meshChromosome Aberrations --chemically induceden_US
dc.subject.meshHumansen_US
dc.subject.meshLymphocytes --drug effectsen_US
dc.subject.meshMutagens --pharmacologyen_US
dc.subject.meshOcimum --chemistryen_US
dc.subject.meshPlant Preparations --pharmacologyen_US
dc.subject.meshSister Chromatid Exchange --drug effectsen_US
dc.titlePossible modulating action of plant infusion of Ocimum sanctum L. on chromosomal aberrations and sister chromatid exchanges induced by chlormadinone acetate in human lymphocytes in vitro.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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