Duodenal mucosal immune cells in treatment-naive adult patients with celiac disease having different histological grades and controls

Simple item page
dc.contributor.authorS.Gahlot, GPen_US
dc.contributor.authorDas, Pen_US
dc.contributor.authorBaloda, Ven_US
dc.contributor.authorSingh, Aen_US
dc.contributor.authorVishnubhatla, Sen_US
dc.contributor.authorGupta, SDen_US
dc.contributor.authorMakharia, GKen_US
dc.date.accessioned2020-04-10T01:50:27Z
dc.date.available2020-04-10T01:50:27Z
dc.date.issued2019-09
dc.description.abstractBackground: It is hypothesized that the duodenal mucosal damage in patients with celiac disease (CeD) is caused by the mucosa-infiltrating lymphoid cells. This study aimed to analyze the immune effective and regulatory T (Treg) cells in duodenal biopsies from treatment-naive adult patients with CeD having different histological grades and controls. Patients and Methods: Dual-color immunohistochemical staining was done in a total of 234 duodenal biopsies, including 132 controls and 102 adult patients with CeD using CD20, CD3:CD4, CD3:CD8, CD4:FoxP3, CD8:FoxP3, and TCR??:TCR?? antibodies. The density of these lymphoid cells in lamina propria and mucosal epithelium was compared between controls and CeD, with different modified Marsh grades. Results: Densities of CD4+ T cells in lamina propria and CD8+?? intraepithelial lymphocytes (IELs) were significantly more in biopsies from patients with CeD, than in controls. An increasing linear pattern of IELs, CD3+ T cells, and CD20+ B cells was observed with increasing grades of villous abnormalities. Although CD8+ FoxP3+ Treg cells were significantly more in biopsies from patients with CeD, there was no significant difference in CD4+ FoxP3+ Treg cell infiltrate between both the groups. Conclusion: Our finding in this observational study generates interest to study the local intestinal mucosal immunity in CeD in detail. A study to prove the failure of CD4+ FoxP3+ Treg cell recruitment in CeD and its direct functional impact may yield valuable information regarding loss of mucosal tolerance.en_US
dc.identifier.affiliationsDepartment of Pathology, AIIMS, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Gastroenterology and Human Nutritions, AIIMS, New Delhi, Indiaen_US
dc.identifier.affiliationsDepartment of Biostatistics, AIIMS, New Delhi, Indiaen_US
dc.identifier.citationS.Gahlot GP, Das P, Baloda V, Singh A, Vishnubhatla S, Gupta SD, Makharia GK. Duodenal mucosal immune cells in treatment-naive adult patients with celiac disease having different histological grades and controls. Indian Journal of Pathology and Microbiology. 2019 Sep; 62(3): 399-404en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/196391
dc.languageenen_US
dc.publisherIndian Association of Pathologists and Microbiologistsen_US
dc.relation.issuenumber3en_US
dc.relation.volume62en_US
dc.source.urihttps://dx.doi.org/10.4103/IJPM.IJPM_703_18en_US
dc.subjectDuodenumen_US
dc.subjectimmunohistochemistryen_US
dc.subjectintraepithelial lymphocytesen_US
dc.subjectmucosaen_US
dc.subjecttoleranceen_US
dc.titleDuodenal mucosal immune cells in treatment-naive adult patients with celiac disease having different histological grades and controlsen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijpm2019v62n3p399.pdf
Size:
1.53 MB
Format:
Adobe Portable Document Format
Download
Collections
Indian Journal of Pathology & Microbiology