Analogue-resistant mutants of Azotobacter chroococcum derepressed for nitrogenase activity and early ammonia excretion having potential as inoculants for cereal crops.

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dc.contributor.authorLakshminarayana, Ken_US
dc.contributor.authorShukla, Ben_US
dc.contributor.authorSindhu, S Sen_US
dc.contributor.authorKumari, Pen_US
dc.contributor.authorNarula, Nen_US
dc.contributor.authorSheoran, R Ken_US
dc.date.accessioned2009-05-28T13:16:33Z
dc.date.available2009-05-28T13:16:33Z
dc.date.issued2000-04-24en_US
dc.description.abstractSpontaneous mutants resistant to methionine sulfoximine (Msx), methyl alanine (Mal) and methyl ammonium chloride (Mac) were derived from A. chroococcum strain A103. Msx and Mal-resistant mutants expressed 1.73 to 10.98% of the fully derepressed nitrogenase activity when grown in Burk's medium containing ammonium acetate. Mac-resistant mutants did not express nitrogenase activity in ammonium acetate supplemented medium. The mutants excreted ammonia even after 2 days of growth and some mutants excreted more ammonia as compared to the parent. Selected mutants were inoculated on wheat (Triticum aestivum) and barley (Hordeum vulgare) under field conditions. Majority of the derepressed mutants increased grain yield of wheat and barley varying from 1.2 to 33.3%. However, host-dependent effects on grain yield were observed with different mutants. Two mutants, Mal 27 and Mac 19 showed significant increase in grain yields of both the crops. The results suggest that metabolic analogue-resistant mutants of Azotobacter have potential for use as a biofertilizer for cereal crops.en_US
dc.description.affiliationDepartment of Microbiology, CCS Haryana Agricultural University, Hisar 125 004, India. hau@hry.ren.nic.inen_US
dc.identifier.citationLakshminarayana K, Shukla B, Sindhu SS, Kumari P, Narula N, Sheoran RK. Analogue-resistant mutants of Azotobacter chroococcum derepressed for nitrogenase activity and early ammonia excretion having potential as inoculants for cereal crops. Indian Journal of Experimental Biology. 2000 Apr; 38(4): 373-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/59195
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAlanine --analogs & derivativesen_US
dc.subject.meshAmmonia --metabolismen_US
dc.subject.meshAzotobacter --drug effectsen_US
dc.subject.meshCereals --microbiologyen_US
dc.subject.meshDrug Resistance, Microbial --geneticsen_US
dc.subject.meshMethionine Sulfoximine --pharmacologyen_US
dc.subject.meshMethylamines --pharmacologyen_US
dc.subject.meshMutationen_US
dc.subject.meshNitrogen Fixationen_US
dc.subject.meshNitrogenase --geneticsen_US
dc.titleAnalogue-resistant mutants of Azotobacter chroococcum derepressed for nitrogenase activity and early ammonia excretion having potential as inoculants for cereal crops.en_US
dc.typeJournal Articleen_US
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