Adiponectin inhibits vascular smooth muscle cell calcification induced by beta-glycerophosphate through JAK2/STAT3 signaling pathway

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dc.contributor.authorLu, Yanen_US
dc.contributor.authorMa, Yichaoen_US
dc.contributor.authorWang, Ruihuaen_US
dc.contributor.authorSun, Jingen_US
dc.contributor.authorGuo, Beibeien_US
dc.contributor.authorWei, Ruipengen_US
dc.contributor.authorJia, Yongpingen_US
dc.date.accessioned2020-11-18T10:14:30Z
dc.date.available2020-11-18T10:14:30Z
dc.date.issued2019-09
dc.description.abstractRadioresistance is a material obstacle for effective treatment of colorectal cancer (CRC). Thus, the discovery of a novelbiomarker for determining the CRC radiosensitivity is necessary. Recent studies have confirmed that miR-183-3p regulatescell phenotypes and tumor growth in various cancers. However, the role and mechanism of this micro-ribonucleic acid inCRC radiosensitivity remains unclear. Here, the abundances of miR-183-5p and ATG5 mRNA were detected by a real-timequantitative reverse transcription polymerase chain reaction. Kaplan–Meier survival analysis was carried out to explore thecorrelation between miR-183-5p and patient prognosis. Cell viability was evaluated by the MTT assay. Survival fractionanalysis through colony formation was performed to assess the cell radiation response. Bioinformatic, luciferase andwestern blot assays were employed to verify the targeted interaction between miR-183-5p and ATG5. The results showedthat an elevated abundance of miR-183-5p and a reduced ATG5 level in CRC were associated with the poor prognosis. Theknockdown of miR-183-5p enhanced the sensitivity of CRC cells to radiation, inflected by the decreased cell viability andsurvival fraction. Mechanically, ATG5 was targeted by miR-183-5p. The addition of ATG5 conferred the radiosensitivity ofthe CRC cells, which was revered by miR-183-5p restoration. Furthermore, miR-183-5p knockdown hindered the tumorgrowth by repressing ATG5 in vivo after radiation treatment. In summary, the output data indicated that miR-183-5pheightened the radiation response of the CRC cells by targeting ATG5, promising a novel therapeutic target for CRCpatients with radioresistance.en_US
dc.identifier.affiliationsDepartment of Cardiology, The First Hospital of Shanxi Medical University, No. 85 Jiefangnan Road, Taiyuan 030001, Shanxi, People’s Republic of Chinaen_US
dc.identifier.affiliationsDepartment of Cardiology, The First Hospital of Shanxi Medical University, No. 85 Jiefangnan Road, Taiyuan 030001, Shanxi, People’s Republic of Chinaen_US
dc.identifier.affiliationsDepartment of Cardiology, The First Hospital of Shanxi Medical University, No. 85 Jiefangnan Road, Taiyuan 030001, Shanxi, People’s Republic of Chinaen_US
dc.identifier.affiliationsDepartment of Cardiology, The First Hospital of Shanxi Medical University, No. 85 Jiefangnan Road, Taiyuan 030001, Shanxi, People’s Republic of Chinaen_US
dc.identifier.affiliationsDepartment of Cardiology, The First Hospital of Shanxi Medical University, No. 85 Jiefangnan Road, Taiyuan 030001, Shanxi, People’s Republic of Chinaen_US
dc.identifier.affiliationsDepartment of Cardiology, The First Hospital of Shanxi Medical University, No. 85 Jiefangnan Road, Taiyuan 030001, Shanxi, People’s Republic of Chinaen_US
dc.identifier.affiliationsShanxi Medical University, No. 56 Xinjiannan Road, Taiyuan 030001, Shanxi, People’s Republic of Chinaen_US
dc.identifier.citationLu Yan, Ma Yichao, Wang Ruihua, Sun Jing, Guo Beibei, Wei Ruipeng, Jia Yongping. Adiponectin inhibits vascular smooth muscle cell calcification induced by beta-glycerophosphate through JAK2/STAT3 signaling pathway. Journal of Biosciences. 2019 Sep; 44(4): 1-9en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214430
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.issuenumber4en_US
dc.relation.volume44en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-019-9895-1en_US
dc.subjectAdiponectinen_US
dc.subjectJAK2/STAT3en_US
dc.subjectvascular calcificationen_US
dc.subjectvascular smooth muscleen_US
dc.titleAdiponectin inhibits vascular smooth muscle cell calcification induced by beta-glycerophosphate through JAK2/STAT3 signaling pathwayen_US
dc.typeJournal Articleen_US
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