Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand.

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dc.contributor.authorPrapanthadara, Len_US
dc.contributor.authorKoottathep, Sen_US
dc.contributor.authorPromtet, Nen_US
dc.contributor.authorSuwonkerd, Wen_US
dc.contributor.authorKetterman, A Jen_US
dc.contributor.authorSomboon, Pen_US
dc.date.accessioned2009-05-27T16:19:45Z
dc.date.available2009-05-27T16:19:45Z
dc.date.issued2000-03-21en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractComparative DDT-susceptibility status as well as glutathione S-transferase activity and DDTase activity of Anopheles minimus (A). An. annularis and Culex quinquefasciatus were investigated to ascertain the role of these enzymes in DDT-resistance. The standard WHO susceptibility test kits was used to discriminate between resistant and susceptible populations. GST activity was measured in microtiter plates whereas DDTase activity was determined by HPLC quantitation of DDT metabolites. This is the first report of DDT-resistance in the Thai malaria vector, An. minimus species A. A positive correlation of DDT-resistance and DDTase activity was observed in this species as well as in the suspected vector, An. annularis. However, GST activity was not correlated to DDT-resistance in either species. Statistical analysis and scatter plots demonstrated the non-correlation between DDTase and GST activity in An. annularis. Studies in Culex quinquefisciatus revealed difference in GST/ DDTase and the relationship to DDT-resistance compared to the anopheline species. The Culex GST activity is correlated to DDTase activity. These results suggested that a positive correlation of GST and DDTase activity might be species dependent.en_US
dc.description.affiliationResearch Institute for Health Sciences, Chiang Mai University, Thailand.en_US
dc.identifier.citationPrapanthadara L, Koottathep S, Promtet N, Suwonkerd W, Ketterman AJ, Somboon P. Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 2000 ; 31 Suppl 1(): 111-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34958
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnopheles --enzymologyen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshCulex --enzymologyen_US
dc.subject.meshDDT --metabolismen_US
dc.subject.meshGlutathione Transferase --metabolismen_US
dc.subject.meshLinear Modelsen_US
dc.subject.meshLyases --metabolismen_US
dc.subject.meshSpecies Specificityen_US
dc.subject.meshThailanden_US
dc.titleCorrelation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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