A preliminary study on in vitro transmission of Dirofilaria immitis infective stage larvae by Aedes aegypti (L.) (Diptera: Culicidae).

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dc.contributor.authorTiawsirisup, Sonthayaen_US
dc.contributor.authorKhlaikhayai, Thodsathamen_US
dc.contributor.authorNithiuthai, Suwanneeen_US
dc.date.accessioned2009-05-27T16:04:23Z
dc.date.available2009-05-27T16:04:23Z
dc.date.issued2005-11-28en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThis study was performed to study an in vitro transmission of infective stage larvae from the mosquito proboscis. There were five experiments with 949 mosquitoes. Liverpool strain of Aedes aegypti (L.) were used in this study. They were allowed to feed on D. immitis infected dogs with different microfilarial levels which were 1,650, 1,950, 9,000, 9,250, and 11,550 microfilariae per one ml of blood. Mosquitoes were forced to feed on solution (5% sucrose in 5% dog serum) in capillary tubes for 20 minutes at 7-34 days post-blood feeding. Solutions in capillary tubes then were examined and mosquitoes were dissected and examined for D. immitis larvae under a light microscope. Second stage larvae could be found in the abdomen and malpighian tubules of mosquitoes and third stage larvae can be found in the abdomen, malpighian tubules, thorax, and proboscis of mosquitoes with different levels of infection. No larvae were detected in the solution in capillary tubes of all experiments.en_US
dc.description.affiliationVeterinary Parasitology Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn Univesity, Henry Dunant Road, Bangkok 10330, Thailand.en_US
dc.identifier.citationTiawsirisup S, Khlaikhayai T, Nithiuthai S. A preliminary study on in vitro transmission of Dirofilaria immitis infective stage larvae by Aedes aegypti (L.) (Diptera: Culicidae). The Southeast Asian Journal of Tropical Medicine and Public Health. 2005 ; 36 Suppl 4(): 86-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34351
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/2005_36_spp4/36sup4_86.pdfen_US
dc.subject.meshAedes --parasitologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCulicidae --parasitologyen_US
dc.subject.meshDirofilaria immitis --pathogenicityen_US
dc.subject.meshDirofilariasis --parasitologyen_US
dc.subject.meshDogsen_US
dc.subject.meshHumansen_US
dc.subject.meshInsect Vectorsen_US
dc.subject.meshLarvaen_US
dc.titleA preliminary study on in vitro transmission of Dirofilaria immitis infective stage larvae by Aedes aegypti (L.) (Diptera: Culicidae).en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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